Method for carrying out secondary complexing precipitation and purification on sodium hyaluronate

A technology of complexation precipitation and sodium hyaluronate, which is applied in the field of sodium hyaluronate secondary complexation precipitation purification, can solve the problems of incomplete complexation precipitation, viscous supernatant, and high ionic strength

Inactive Publication Date: 2012-06-27
SHANGHAI JINGFENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 1. The ionic strength is high or low, and the complexation and precipitation are not complete, which affects the yield
[0008] 2. The ionic strength is abnormal, resulting in poor properties of the complexed precipitate, the supernatant is viscous, and it is difficult to collect the complexed precipitate
[0009] The above method will cause unnecessary waste of sodium hyaluronate in the purification process and reduce production efficiency; it will make it difficult to empty the supernatant in actual large-scale production, increase energy consumption, and is not conducive to large-scale production operations

Method used

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  • Method for carrying out secondary complexing precipitation and purification on sodium hyaluronate
  • Method for carrying out secondary complexing precipitation and purification on sodium hyaluronate
  • Method for carrying out secondary complexing precipitation and purification on sodium hyaluronate

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Experimental program
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Effect test

Embodiment 1

[0025] Sodium hyaluronate was prepared by the existing extraction method, and 2400ml of the filtrate was taken from the cockscomb enzymatic hydrolysis solution once activated carbon was adsorbed and filtered, and divided into 6 parts on average, numbered A, B, C, D, E, F respectively. Use purified water or sodium chloride to adjust the conductivity of the filtrate to the following: A-600us / cm, B-1.2ms / cm, C-1.8ms / cm, D-2.4ms / cm, E-3.0ms / cm, F-3.6ms / cm, respectively take 1.6g of CPC and add appropriate amount of water to dissolve, then complex and precipitate with the above 6 filtrates under the same conditions, collect the precipitate after complexation, and prepare 260ml of 0.4mol / L solution The complexed precipitate was dissociated by the chaotrope for about 5 hours, and then the HA was alcohol-precipitated with 2 times the volume of 95% ethanol, dehydrated three times, and then vacuum-dried to obtain HA, and the yield was compared.

Embodiment 2

[0027] Sodium hyaluronate was prepared by the existing extraction method, and 2000ml of the filtrate was taken from the cockscomb enzymatic hydrolysis solution once activated carbon was adsorbed and filtered, and divided into 6 parts on average, numbered A, B, C, D, E, F respectively. Use purified water or sodium chloride to adjust the conductivity of the filtrate to the following: A-600us / cm, B-1.2ms / cm, C-1.8ms / cm, D-2.4ms / cm, E-3.0ms / cm, F-3.6ms / cm, respectively take 1.7g of CPC and add appropriate amount of water to dissolve, then complex and precipitate with the above 6 filtrates under the same conditions, collect the precipitate after complexation, and prepare 230ml of 0.4mol / L solution The complexed precipitate was dissociated by the chaotrope for about 5 hours, and then the HA was alcohol-precipitated with 2 times the volume of 95% ethanol, dehydrated three times, and then vacuum-dried to obtain HA, and the yield was compared.

Embodiment 3

[0029] Sodium hyaluronate was prepared by the existing extraction method, and 4800ml of the filtrate was taken from the cockscomb enzymatic hydrolysis solution once activated carbon adsorption and filtered, and divided into 6 parts on average, numbered A, B, C, D, E, F respectively. Use purified water or sodium chloride to adjust the conductivity of the filtrate to the following: A-600us / cm, B-1.2ms / cm, C-1.8ms / cm, D-2.4ms / cm, E-3.0ms / cm, F-3.6ms / cm, respectively take 3.2g of CPC and add appropriate amount of water to dissolve, then complex and precipitate with the above 6 filtrates under the same conditions, collect the precipitate after complexation, and prepare 520ml of 0.4mol / L solution The complexed precipitate was dissociated by the chaotrope for about 5 hours, and then the HA was alcohol-precipitated with 2 times the volume of 95% ethanol, dehydrated three times, and then vacuum-dried to obtain HA, and the yield was compared. Observe the properties of complexation and p...

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Abstract

The invention relates to a method for carrying out secondary complexing precipitation and purification on sodium hyaluronate, which comprises the following steps of: firstly, adsorbing and filtering a cockscomb enzymatic hydrolysate with activated carbon, then adjusting the ionic strength to carry out complexing precipitation on CPC (Cetylpyridinium Chloride) and the sodium hyaluronate to a maximum limit and precipitating the sodium hyaluronate with ethanol after a complex is dissociated. Through the method for preparing the sodium hyaluronate, the yield of the sodium hyaluronate can be effectively improved, and the production efficiency can be improved.

Description

Technical field: [0001] The invention relates to a purification method of sodium hyaluronate secondary complex precipitation. Background technique: [0002] Hyaluronic acid (HA for short) is composed of (1→3)-2-acetylamino-2-deoxy-β-D-glucose-(1→4)-O-β-D-glucuronic acid bis A linear polysaccharide composed of sugar repeating units with the molecular formula (C 14 h 21 NO 11 )n, according to different tissue sources, the molecular weight range is 2×10 5 ~7×10 6 , the number of disaccharide units is 300 to 11,000 pairs. [0003] [0004] Commercial hyaluronic acid is generally in the form of sodium salt, white fibrous or powdery solid, with strong hygroscopicity, soluble in water, insoluble in organic solvents. The macromolecular network structure of sodium hyaluronate passes through the interaction with H 2 O forms hydrogen bonds to bind a large amount of water, and has the functions of forming various matrices, regulating osmotic pressure, regulating the transport ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 张云陈亮汪洋
Owner SHANGHAI JINGFENG PHARMA
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