Enzyme immobilization technology by film forming through carrier surface phase transformation method
A technology of immobilized enzyme and phase inversion, which is applied in the direction of immobilization on or in the inorganic carrier to achieve the effect of improving activity, fast immobilization speed and easy operation
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example 1
[0019] Example 1: (1) glucose oxidase (GOD) is configured into an enzyme solution with a concentration of 1 mg / mL, acrylic resin (46% solid content) is dissolved in acetone, and configured into a solution of 0.5 g / ml; (2) take the above Add 0.3g of hollow bricks to the prepared 1mL enzyme solution, stir evenly, take out the hollow bricks and dry them at room temperature; (3) After the hollow bricks are dry, add 1mL of acrylic resin solution, stir to make them fully mixed, take out the hollow bricks and place them in a refrigerator at 4°C Dry in the refrigerator; (4) Rinse with distilled water and set aside.
[0020] The performance of immobilized GOD is as follows:
[0021] M
example 2
[0022] Example 2: (1) first activate α-amylase at a constant temperature of 40° C. for 30 minutes and configure it into a 4 mg / ml enzyme solution, dissolve ethyl cellulose with ethyl acetate, and configure it into a 2% solution; (2) Mix 1ml of α-amylase solution with 4mg of porous sponge carrier, and squeeze repeatedly to allow the sponge carrier to fully absorb the enzyme solution. After complete absorption, squeeze the sponge carrier dry and place it in the air to dry; (3) After the sponge carrier is dried, Add 2ml of ethyl cellulose solution, stir to make the two fully mixed, then take out the sponge carrier and place it in a refrigerator at 4°C to dry; (4) Rinse it with distilled water for later use.
[0023] The performance of immobilized α-amylase is as follows:
[0024]
example 3
[0025] Example three: (1) neutral protease is configured into 6 mg / ml enzyme solution, polysulfone is dissolved in dimethylacetamide, and configured into a 5% solution; (2) 1 ml of neutral protease solution is mixed with ceramsite 0.1 g , stir and mix evenly, take out the ceramsite and place it in the air to dry; (3) After the ceramsite is dried, add 1ml of polysulfone solution, stir to make the two fully mixed, then take out the ceramsite and place it in a refrigerator at 4°C to dry; (4) Repeat step (3); (5) Rinse with distilled water for later use.
[0026] The performance of immobilized neutral protease is as follows:
[0027]
PUM
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