Fumonisins B1 aptamer and applications thereof

Abstract

The invention relates to an aptamer combined with high specificity and high affinity of Fumonisins B1 and applications thereof. The aptamer can be used for detection and analysis and separation and concentration of the Fumonisins B1, and has the characteristics of high specificity, high affinity, uniform quality, high stability, short development cycle, low production cost and convenience in use. The invention also relates to sequences derived from aptamer sequences, which comprise modified sequences.

Description

(1) Technical field: [0001] The invention belongs to the field of biotechnology, and relates to the design and preparation of a fumonisin B1 nucleic acid aptamer combined with fumonisin B1 with high specificity and high affinity by using molecular biology technology - SELEX technology and its application. (two) background technology: [0002] Fumonisins (Fumoni sins, FB) are mainly secondary metabolites produced by fungi F. moniliforme and F. proliferatum, which are diester compounds of polyhydric alcohols and glycerin tricarboxylic acid, which exist in a wide range from millet to grains Fruits such as bananas can be contaminated and exist in various links such as processing, storage, and transportation. Through grain processing, feed production, and other processes, it will cause serious harm to animal husbandry and even human health. Fumonisins mainly include fumonisins B1, B2 and B3, of which more than 60% are fumonisins B1 (FB1). FB1 is the most toxic fumonisins. Isolat...

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Problems solved by technology

Although the aforementioned methods have high sensitivity and specificity, they require complex purification steps and expensive instruments and equipment, and are not easy to be popularized and used; ELISA detection technology based on monoclonal antibodies or polyclonal antibodies has the characteristics of simple, fast and sensitive , can detect a large number of samples at the same time, compared with the HPLC method and LC-MS method, it has the advantages of high sensitivity, less interference, simple and fast determination steps, safe operation, less equipment investment, and accurate and reliable determination results

However, for FB, a toxic small molecule, the preparation cycle of the antibody is long, the production cost is high, the technology is difficult, the batch-to-batch variation is large, and the clone (cell) is difficult to effectively preserve, which greatly limits the ability of enzyme-linked immunosorbent assay, etc. The rapid development of immunological detection technology

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