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Method for simultaneous quantitative detection of zearalenone and fumonisin B1

A technology of zearalenone and fumonisins, which is applied in the directions of measuring devices, instruments, scientific instruments, etc., can solve the problems of not being able to detect two toxins at the same time, affecting the detection of mycotoxins, uneconomical and environmental protection, etc. The effect of grassroots promotion and application, fast detection speed and short time required

Inactive Publication Date: 2012-06-27
SHANGHAI JIAO TONG UNIV
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Problems solved by technology

Although there are currently detection kits for zearalenone and fumonisin B1, each detection kit can only detect one toxin, and cannot detect two toxins at the same time. The operation is troublesome, uneconomical and environmentally friendly. , has seriously affected the detection of mycotoxins in actual work. The present invention will fundamentally provide a fast and simple quantitative detection method, and provide a fast one-step quantitative detection method for entry-exit quarantine, customs, production enterprises, supervision departments and other units Zearalenone and Fumonisin B1

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  • Method for simultaneous quantitative detection of zearalenone and fumonisin B1
  • Method for simultaneous quantitative detection of zearalenone and fumonisin B1
  • Method for simultaneous quantitative detection of zearalenone and fumonisin B1

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Embodiment

[0032] 1. Antigen preparation

[0033] (1) Preparation of zearalenone-coated antigen

[0034] Take 0.33ml (3mg / ml) ZEN, mix it in 1.2ml pyridine, add 2mg O-carboxymethyl hydroxylamine, stir and react at room temperature for 24h; after vacuum drying the solution, add 4ml distilled water and dissolve it, adjust the pH to 8.0 ; The unreacted ZEN was extracted and removed with benzene (3ml benzene, extracted 3 times in total), the benzene phase was removed, and the water phase was retained. The pH of the aqueous phase was adjusted to 3.0, extracted with ethyl acetate (10 ml ethyl acetate, extracted 4 times in total), the ester phase was extracted, and the aqueous phase was discarded. The ester phase was filtered with anhydrous sodium sulfate and dried. The dried crystals were dissolved in 0.5ml of basic alumina-treated dioxane; 20mg of BSA was weighed and dissolved in 0.7ml of 0.05mol / L (pH7.2) PBS; after that, the two solutions were dissolved in Mix slowly at 4°C to obtain sol...

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Abstract

The invention relates to a method for simultaneous quantitative detection of zearalenone and fumonisin B1. The method comprises the steps of: 1. preparation of conjugates ZEN-BSA and ZEN-OVA, FB1-KLH and FB1-OVA; 2. preparing an anti-ZEN monoclonal antibody and an anti-FB1monoclonal antibody; 3. preparing colloidal gold, labelling the monoclonal antibodies, and spraying the labelled monoclonal antibodies to a gold labeled pad; 4. conducting sample application on a cellulose nitrate membrane; 5. carrying out assembling to obtain a test strip; and 6. respectively drawing standard curves of the concentrations and color values of ZEN and FB1, bringing the color value of a sample to be tested into the standard curves so as to obtain the content of ZEN and FB1 in the sample to be tested. The method of the invention has the advantages of high detection accuracy, fast detection speed, and short detection time, and can be used without training, thus meeting the demands for rapid and correct judging of ZEN and FB1 content of grain storage and sales organizations, as well as immigration, customs and other inspection departments.

Description

technical field [0001] The invention relates to a method in the technical field of biological detection, in particular to a method for quantitatively detecting zearalenone and fumonisin B1 simultaneously. Background technique [0002] Zearalenone toxin (Zearalenone, ZEN) is a secondary metabolite produced by some strains of Fusarium under certain humidity and temperature conditions. Researchers have detected zearalenone in grains such as wheat, barley, corn, rye, and sorghum, and also in some animal tissues or products, including milk, eggs, etc. Zearalenone toxin can cause a variety of diseases in humans and animals, mainly carcinogenic and cancer-promoting toxicity. Reproductive system diseases have an important relationship. Zearalenone toxin has the characteristics of widespread distribution, long residual time, difficult to handle, and enhanced toxicity with other toxins. Fumonisin B1 (Fumonisin B1, FB1) is a secondary metabolite produced by Fusarium moniliforme unde...

Claims

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Application Information

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IPC IPC(8): G01N33/577G01N33/569G01N33/558
Inventor 严亚贤王元凯孙建和
Owner SHANGHAI JIAO TONG UNIV
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