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Oligopeptide with breast cancer resistant activity and application thereof

An anti-breast cancer and breast cancer cell technology, applied in oligopeptides and their applications, oligopeptides with anti-breast cancer activity and their applications, can solve the problems of long cycle and high cost, and achieve growth inhibition, small molecular weight, and inhibition proliferating effect

Inactive Publication Date: 2012-07-04
ZHUHAI JINLONG BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] In the early stage, bioactive peptides were mainly isolated and expanded from natural animals, plants, insects and other organisms, with long cycle and high cost

Method used

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  • Oligopeptide with breast cancer resistant activity and application thereof
  • Oligopeptide with breast cancer resistant activity and application thereof
  • Oligopeptide with breast cancer resistant activity and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Design and synthesis of oligopeptides

[0030] 1. Extract the major international antimicrobial peptide database (http: / / aps.unmc.edu / AP / main.php; http: / / penbase.immunaqua.com / ; http: / / www.bbcm.univ.trieste.it / ; Http: / / www.imtech.res.in / raghava / antibp / ), analyze the corresponding relationship between its primary structure and activity, and design the artificial short peptide sequence.

[0031] 2. Use the protein analysis database http: / / www.expasy.org / to analyze the similarity between the oligopeptide sequence of known activity and the secondary structure of the designed oligopeptide sequence, and combine other physical and chemical parameter predictions to modify the design sequence .

[0032] 3. Send the oligopeptide sequence to the biological company for synthesis or use an automatic oligopeptide synthesizer to synthesize the full sequence. HPLC detection purity is greater than 96%, and mass spectrometry is used to determine the quality of oligopeptide synthesis. The s...

Embodiment 2

[0037] Identification of oligopeptides

[0038] P1221 oligopeptide was hydrolyzed by 5.7mol / L HCl for 16 hours and analyzed for amino acid. It was found that it contained 9 kinds of amino acids, including His, Asn, Tyr, Gly, Phe, Val, Pro, Ser, Leu, and its molar ratio was His:Asn : Tyr: Gly: Phe: Val: Pro: Ser: Leu = 1: 1: 1: 1: 1: 1: 1: 1: 1 After amino acid sequence analysis, the sequence is His-Asn-Tyr-Gly-Phe-Val-Pro-Ser-Leu. A peak was determined by electrospray mass spectrometry, and its molecular weight was 1033.15.

[0039]

Embodiment 3

[0041] Detection of tumor suppressor activity of P1221 oligopeptide in vitro by MTT method

[0042] 2.1 Materials and reagents

[0043] 1) P1221 oligopeptide, synthesized by Shanghai Shenggong Biological Engineering Technology Service Co., Ltd.

[0044] 2) Human breast cancer cell lines MCF-7, MDA-MB-231, and human normal breast cells MCF-10A were purchased from the Cell Resource Center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

[0045] 3) RPMI 1640 medium, DMEM medium, and newborn calf serum are all products of GIBCO, USA.

[0046] 4) Dimethyl sulfoxide (DMSO), tetramethyl azoazole (MTT) and trypsin are products of Sigma.

[0047]

[0048] 2.2 Experiment

[0049] 1) Dilute P1221 oligopeptide to an initial concentration of 1mM with diluent (sterile PBS, or sterile water, or corresponding cell culture medium), filter and sterilize, and freeze at -20°C after packaging.

[0050] 2) Cell culture: The cancer cells are cultured in RPMI 1640 culture medium (contain...

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Abstract

The invention discloses an oligopeptide with breast cancer resistant activity. The oligopeptide has a sequence of His-Asn-Tyr-Gly-Phe-Val-Pro-Ser-Leu. The oligopeptide can selectively inhibit the growth of in-vitro breast cancer cells under low concentration and does not have any influence on normal breast cells. In-vivo experiments show that the oligopeptide has a strong effect of inhibiting the growth and proliferation of cancer cells and has an obvious dose-effect relationship. Because of a safe, efficient and ideal anti-cancer effect, the oligopeptide can be used for preparing anti-cancer medicines and has a good application prospect.

Description

[0001] technical field [0002] The present invention relates to oligopeptide and its application, in particular to an oligopeptide with anti-breast cancer activity and its application, which belongs to the field of biomedicine. [0003] Background technique [0004] Breast cancer is the main malignant tumor that endangers women's health. About 1.2 million women in the world develop breast cancer every year, accounting for 18% of all tumors in women. The 5-year survival rate of breast cancer is about 50%-60%, nearly 50% of patients relapse and metastasize after treatment, and the average survival time of patients with advanced breast cancer is only 18-30 months. Breast cancer treatment methods include surgery, radiotherapy, chemotherapy, endocrine therapy and other traditional treatments. However, the risk of surgical treatment is high, the trauma to the human body is large, the patient's immunity is reduced, and the resistance to the disease is reduced. A series of surgic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06A61K38/08A61P35/00
Inventor 胡海波
Owner ZHUHAI JINLONG BIOTECH CO LTD
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