Method for purifying lipstatin

A purification method, the technology of riprex, which is applied in the field of riprestatin purification, can solve the problems of health hazards of operators, large loss of target products, and many steps in the extraction process, and achieve high extraction rate, low cost, and few times Effect

A purification method, the technology of riprex, which is applied in the field of riprestatin purification, can solve the problems of health hazards of operators, large loss of target products, and many steps in the extraction process, and achieve high extraction rate, low cost, and few times Effect

CN102558104AInactive Publication Date: 2012-07-11PEKING UNIV FOUNDER GRP CO LTD +2
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  • Method for purifying lipstatin
  • Method for purifying lipstatin

Examples

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Embodiment 1

[0180] When the fermentation broth is directly spray-dried, a centrifugal nozzle spray dryer is used. The gas inlet temperature of the spray dryer is controlled at 40°C, the gas outlet temperature is controlled at 35°C, the temperature of the material in the tower is kept at 40°C, and the pressure in the tower is kept at 250kPa to obtain solid fermented dry bacteria powder with a particle size of 50 mesh.

[0181] Put the above fine powder into supercritical CO 2 In the extraction kettle, adjust the extraction temperature, pressure, time, control CO 2 Flow rate for extraction, wherein: extraction kettle temperature 35°C, pressure 40.0MPa, time 4h, CO 2 The flow rate is 30L / h. The entrainer ethanol (the ratio of the volume of the entrainer to the weight of the fine powder is 5:1 (L:Kg)) was added to the extraction kettle, and its flow rate was set at 4 L / h. Then set the separation temperature of the first separation tank to 30° C. and the separation pressure to 8 MPa for the...

Embodiment 2

[0190] Spray-dry the fermentation broth directly using a centrifugal nozzle spray dryer. The gas inlet temperature of the spray dryer is controlled at 60°C, the gas outlet temperature is controlled at 50°C, the temperature of the material in the tower is kept at 55°C, and the pressure in the tower is kept at 350kPa , to obtain solid fermented and dried bacteria powder with a particle size of 80 mesh.

[0191] Put the above fine powder into supercritical CO 2 In the extraction kettle, adjust the extraction temperature, pressure, time, control CO 2 Flow rate for extraction, wherein: extraction kettle temperature 60°C, pressure 20.0MPa, time 2h, CO 2 The flow rate is 10L / h. The entrainer ethanol (the ratio of the volume of the entrainer to the weight of the fine powder is 3:1 (L:Kg)) was added into the extraction kettle, and its flow rate was set at 2 L / h. Then set the separation temperature of the first separation tank to 50° C. and the separation pressure to 5.5 MPa for the ...

Embodiment 3

[0200] The fermentation liquid is directly spray-dried using a centrifugal nozzle spray dryer. The gas inlet temperature of the spray dryer is controlled at 50°C, the gas outlet temperature is controlled at 40°C, the temperature of the material in the tower is kept at 45°C, and the pressure inside the tower is kept at 300kPa , to obtain solid fermented and dried bacteria powder with a particle size of 40 mesh.

[0201] Put the above fine powder into supercritical CO 2 In the extraction kettle, adjust the extraction temperature, pressure, time, control CO 2 Flow rate for extraction, wherein: the temperature of the extraction kettle is 40°C, the pressure is 30.0MPa, the time is 3h, CO 2 The flow rate is 30L / h. The entrainer ethanol (the ratio of the volume of the entrainer to the weight of the fine powder is 3:1 (L:Kg)) was added into the extraction kettle, and its flow rate was set at 2 L / h. Then set the separation temperature of the first separation tank to 40° C. and the s...

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Abstract

The invention provides a method for purifying lipstatin. The method comprises the following steps of: carrying out extraction on a solid mixture containing the lipstatin by using supercritical CO2 fluid, and then, purifying an obtained lipstatin extract to obtain the lipstatin. The method provided by the invention has the characteristics of high efficiency, environment-friendliness, simple process, high purity of finished products, high yield and the like.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a method for purifying liprestatin. Background technique [0002] Lipstatin is a metabolite of Streptomyces toxytricini, which can selectively inhibit the activity of pancreatic lipase in the gastrointestinal tract and reduce the decomposition and absorption of fat. Its structural formula is shown in formula I. Its tetrahydro derivative orlistat (orlistat) has been successfully developed by Roche Inc. (Roche lnc.) as a weight loss drug - Xenical, which is currently the only drug for the treatment of obesity marketed as a non-central nervous system effect. Therefore, the production of riprestatin by fermentation has attracted much attention. [0003] [0004] The production process of liprestatin through microbial biosynthesis mainly includes the following steps: the microbial fermentation broth is directly crushed by ultrasonic waves, extracted with an organic solvent, and then concen...

Claims

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Application Information

Patent Timeline
11 Jul 2012
Publication
CN102558104A
IPC
C07D305/12
CPC
Y02P20/54
Inventors
赵德; 岳光