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Pyrene-degrading bacterial strain

A strain and function technology, applied in the field of biodegradation treatment of contaminated soil and water, can solve the problems of lack of efficient polycyclic aromatic hydrocarbon degrading bacteria, restricting development and application, etc.

Inactive Publication Date: 2014-03-05
BEIJING NORMAL UNIVERSITY
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Problems solved by technology

[0003]However, due to the lack of efficient PAH-degrading bacteria, the further development and application of PAH-contaminated soil bioremediation technology is restricted

Method used

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Examples

Experimental program
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Embodiment Construction

[0019] Adding petroleum-contaminated soil to the open glass bottle with pyrene and basal medium for enrichment and domestication of pyrene-degrading microorganisms. Insert the enriched acclimatized soil into the shaped bottle. After culturing in an oscillator at a temperature of 25-30°C and a rotation speed of 100r / min for 5-7 days, the cycle transfer is carried out, and after the number of cycles is more than 8 times, a degrading bacterial group that can efficiently remove pyrene can be obtained. Make the bottom plate of agar solid medium with pyrene as the only carbon source and energy source in the ultra-clean workbench, make the top plate of agar solid medium containing pyrene-degrading bacteria on the bottom plate, and place the prepared medium plate in the incubator Cultured in medium for 10-15 days. In the ultra-clean workbench, insert the colony on the medium plate into the conical flask with pyrene, basal medium, trace metal liquid, vitamin C solution and adsorbent, ...

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Abstract

The invention belongs to the technical field of biological degradation, and particularly discloses a pyrene-degrading bacterial strain which is identified to be Stenotrophomonas acidaminiphila, and the culture preservation number is CGMCC3683. The bacterial strain is prepared into bacterial suspension with bacterial content of 1.25*108CFU / ml, and has the degradation removal rates of 96.31%, 92.55%,90.17% and 88.48% on the pyrene with the initial concentration of 1.03mg / L, 0.29mg / L, 0.58mg / L and 1.21mg / L after 7 days.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, and particularly relates to a bacterial strain capable of degrading pyrene. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of condensed ring compounds containing two or more benzene rings arranged in linear, angular or clustered shapes, with hydrophobicity, low solubility, low vapor pressure, High melting point and boiling point, high octanol-water partition coefficient and other characteristics. Polycyclic aromatic hydrocarbons have strong carcinogenic, teratogenic and mutagenic effects. Generally speaking, low-molecular-weight PAHs with 2-3 rings have significant acute toxicity, while some high-molecular-weight PAHs have potential carcinogenicity. Due to the presence of conjugated large π-bond electron systems in PAHs molecules, they have high stability, their photolysis and biodegr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00B09C1/10
Inventor 程莉蓉豆俊峰丁爱中杜勇超李帅冉范福强
Owner BEIJING NORMAL UNIVERSITY
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