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Method for rapidly enriching organic selenium on spirulina

A technology of organic selenium and spirulina, applied in the direction of microorganism-based methods, biochemical equipment and methods, single-cell algae, etc., can solve the problems of easy loss, waste, and low utilization rate, and achieve convenient operation and improve absorption and transformation rate, good effect

Active Publication Date: 2012-07-11
LIJIANG CHENGHAI BAOER BIOLOGICAL DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, ionic selenium dissolved in water is particularly easy to lose during the production process, resulting in low utilization rate and insignificant economic benefits
If you often ingest high levels of ionic selenium, it will not only cause waste, but also cause selenium poisoning.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] In a clean cement pool with an area of ​​400 square meters, add fresh water with a water depth of 0.25m. In a 10-ton water tank, add 2000g NaHCO to 1 ton of water 3 , 2000g sodium nitrate, 2000g urea, 500g potassium dihydrogen phosphate, 15g ferric chloride, 1.5g manganese chloride and 2g each of vitamin B1 and B12, add while stirring until completely dissolved, and pour into the culture tank, inoculate fresh spiral Algae species 1500kg. In the evening of the day when organic selenium is enriched, start to add sodium selenite, add 30g of sodium selenite to 1 ton of water, repeat the next evening, and the suitable temperature is 30 degrees. After each addition of the medium, the water truck stirred for 5 hours, the flow rate was controlled at 1.0 m / s, and stood for 5 hours; the fresh spirulina was harvested before dawn on the third day, sprayed and dried after cleaning, so that the selenium of spirulina The content reaches 600μg / g.

Embodiment 2

[0016] In a clean cement pool with an area of ​​40 square meters, add fresh water with a water depth of 0.25m. In a 1 ton water tank, add 200g NaHCO to 1 ton of water respectively 3 , 200g sodium nitrate, 200g urea, 50g potassium dihydrogen phosphate, 1.5g ferric chloride, 0.15g manganese chloride and 0.2g each of vitamin B1 and B12, add while stirring until completely dissolved, inject into the culture tank, inoculate fresh Spirulina algae species 150 kg. In the evening of the day when organic selenium is enriched, start to add sodium selenite, add 10 g of sodium selenite to 1 ton of water, repeat the next evening, and the suitable temperature is 25-30 degrees. After each addition of medium, the water truck stirred for 2 hours, the flow rate was controlled at 1.0 m / s, and stood still for 10 hours; the fresh spirulina was harvested before dawn on the third day, and spray-dried after cleaning to make the selenium of spirulina The content reaches 200μg / g.

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Abstract

The invention provides a method for rapidly enriching organic selenium on spirulina. The method comprises the following steps of: respectively adding 200g of NaHCO3, 200g of sodium nitrate, 200g of urea, 50g of potassium dihydrogen phosphate, 1.5g of ferric chloride, 0.15g of manganese chloride, 0.2g of vitamin B1 and 0.2g of vitamin B12 in 1 ton of water, stirring until various components are completely dissolved, injecting an obtained mixture in a culture tank, and inoculating 150kg of fresh spirulina strains; enriching organic selenium, wherein 10-30g of sodium selenite is added in 1 ton of water at night, 10-30g of sodium selenite is repeatedly added at night next day, and after adding each time, stirring is carried out for 2-5 hours with a watercart and then standing is carried out for 5-8 hours; and before dawn on the third day, harvesting fresh spirulina. The method is suitable for rapidly enriching organic selenium on spirulina on a large scale; and the absorption and conversion rates of organic selenium can be obviously improved.

Description

technical field [0001] The invention relates to a method for rapidly enriching organic selenium in spirulina. Background technique [0002] Selenium has a good recovery function for the sub-health of the human body. It can assist in inhibiting tumor growth, anti-oxidation and scavenging free radicals in the body, and relieve or reduce the harm of toxic heavy metals, such as lead, mercury, and arsenic. Selenium deficiency will lead to stagnant growth and development, decreased immunity, prone to symptoms such as senile cataract and myopia. However, the distribution of selenium on the earth is very uneven. More than 70% of the land in my country is in a state of serious selenium deficiency and low selenium, resulting in as many as 22 provinces with selenium deficiency. The recommended per capita food intake is 50-200 Micrograms, especially in harsh environments and increasing work pressure, will induce cancer, liver disease, cardiovascular and cerebrovascular diseases, diabete...

Claims

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Application Information

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IPC IPC(8): C12N1/12C12R1/89
Inventor 徐青山谭应宏彭友邻骆其君严小军殷丽川屈桂华
Owner LIJIANG CHENGHAI BAOER BIOLOGICAL DEV