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Culture medium for quickly propagating caralluma hesperidum

A technology of culture medium and rooting medium, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problem of low reproductive efficiency of Zilongjiao, and achieve the effect of improving process efficiency

Inactive Publication Date: 2012-07-18
YANGTZE UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional propagation efficiency of Zilongjiao is low, and there has been no research report on tissue culture.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] The present invention will be further described below in conjunction with specific examples.

[0029] Utilize the process of rapid propagation of the present invention to cultivate Zilongjiao as follows:

[0030] 1. Preparation of culture medium:

[0031] Prepare the four mediums in proportion respectively, heat and dissolve the prepared medium, adjust its pH value to 6--6.2, put it into a clean cylindrical bottle, seal it and sterilize it under high pressure for about 15 minutes, and set it aside for use .

[0032] 2. Disposal of explants:

[0033] Choose robust growth, with purple-brown markings and plants without purple-brown markings, take a total of 6 explants with axillary bud stems, without axillary bud stems, and spikes, and shake them in a saturated washing powder solution for 5- - After 10 minutes, turn to clean water to rinse and then enter the inoculation room. First use 75% alcohol to sterilize for 5 seconds, then immediately use 0.1% HgCl 2 Sterilize ...

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Abstract

The invention relates to a culture medium for quickly propagating caralluma hesperidum, and belongs to the technical field of plant cells and tissue culture organisms. The culture medium is characterized by comprising four parts, namely an induction callus culture medium, a direct induction cluster bud culture medium, a rejuvenation propagation adventitious bud culture medium and a rooting culture medium; the pH values of the culture media are adjusted to 6 to 6.2; a tissue culture process is divided into four steps, and a corresponding culture medium is used in each step; the culture medium has the characteristics of accurate quantification, simple process, low cost, high propagation speed and high production efficiency; and by the culture method, an integral regeneration system can be established, industrialization production can be realized, the caralluma hesperidum can be quickly propagated and bred, and an isolated culture technology is provided for the development and production of a new steroid medicament.

Description

Technical field: [0001] The invention relates to a culture medium for rapidly propagating Zilongjiao, which belongs to the field of plant cell and tissue culture biotechnology. Background technique: [0002] Zilongjiao is a perennial herbaceous succulent plant in the genus Buffalo in the Astragalus family. It has both ornamental value and precious medicinal plants. In particular, Zilongjiao has thick meat, high yield and rich steroid components, which has great commercial development value. [0003] The domestic and foreign studies on Asclepiaceae plants mainly focus on their chemical constituents, pharmacology, clinical and other aspects. Based on the totipotency of cells, isolated cells of any plant also have the ability of their "mother" plants to synthesize medicinal ingredients under artificial culture. Therefore, by changing and adjusting the in vitro culture conditions, its content and quality can be effectively improved, and then the medicinal components can be ext...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 董社琴杨德良
Owner YANGTZE UNIVERSITY