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Applications of four gypenoside compounds to preparation of medicament for treating tumors

A technology of Gynostemma saponin and compound, applied in the field of Gynostemma japonica leaf extract, can solve the problems of limited source, low yield, low content and the like

Inactive Publication Date: 2012-07-18
MINZU UNIVERSITY OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For some rare saponins with strong activity, the source is limited and the content in the plant is low. Using the traditional separation method not only requires a large amount of medicinal resources, but also the yield is low.

Method used

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  • Applications of four gypenoside compounds to preparation of medicament for treating tumors
  • Applications of four gypenoside compounds to preparation of medicament for treating tumors
  • Applications of four gypenoside compounds to preparation of medicament for treating tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation experiment of embodiment 1 extract

[0043] We compared the extracts prepared by several different preparation methods, and determined the contents of four compounds, Damulin A, Damulin B, gypenoside L, and gypenoside LI. The result is as follows:

[0044] 1. Preparation of Gynostemma pentaphyllum ethanol extract

[0045] The Gynostemma Gynostemma leaves were refluxed and extracted with 8 times the amount (v / w, mL / g) of 80% ethanol for 3 times, 2 hours each time. Concentrate the ethanol extract of Gynostemma pentaphyllum leaves under reduced pressure, and dry to obtain Gynostemma pentaphyllum ethanol extract.

[0046] 2. Preparation of the heat-treated product of Gynostemma pentaphyllum leaf ethanol extract

[0047] Take Gynostemma Gynostemma leaves and use 8 times the amount (v / w, mL / g) of 80% ethanol to reflux extract 3 times, each time for 2h, concentrate, and the concentrated solution is heat-treated at 125°C and 0.24MPa under high temperature and hig...

Embodiment 2

[0057] Embodiment 2 antitumor effect experiment

[0058] 1. Cell lines and cell culture

[0059] Human lung cancer cell A549, human gastric cancer cell MKN45, liver cancer cell SMMC-7721, breast cancer cell MCF-7, leukemia cell HL-60, in DMEM medium containing 10% fetal bovine serum, at 37 ℃, saturated humidity, 5.0 %CO 2 Cultured in an incubator, the cells grew in a monolayer adherent manner. Cells in logarithmic growth phase were used in the experiment.

[0060] 2. MTT drug sensitivity test

[0061] Digest and collect logarithmic growth phase cells and adjust to a concentration of about 1*10 5 / mL single-cell suspension, 100 μL per well was inoculated in a 96-well plate, and after the cells adhered to the wall, various extracts prepared from DMEM culture medium without fetal bovine serum were added. In addition, a blank containing DMEM full medium was also set in the experiment. A control group and a positive control group containing ginseng heat treatment product extra...

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Abstract

The invention relates to a gynostemma pentaphyllum leaf extract which is rich in damulin A, damulin b, gypenoside L and gypenoside LI, and applications of four saponin compounds to preparation of a medicament for treating tumors. Cancers are selected from lung cancer, stomach cancer, liver cancer, breast cancer or leukemia.

Description

technical field [0001] The invention relates to a Gynostemma pentaphyllum leaf extract rich in damulin A, damulin B, gypenoside L and gypenoside LI, and the use of these four saponin compounds in the preparation of medicaments for treating tumors. Background technique [0002] Gynostemma is the whole plant of Gynostemma pentaphyllum (Thunb.) Makino of Cucurbitaceae [1] , has the functions of clearing away heat and detoxification, relieving cough and eliminating phlegm. Also known as "Aescin", "Snake King", "Sweet Tea Man", "Gong Luo Guo Di", "Little Bitter Medicine" and so on. "Compendium of Materia Medica" has the following records on Gynostemma pentaphyllum, "Five leaf vine cures boils, insect bites, cools blood and detoxifies, and facilitates urination". Because Gynostemma pentaphyllum contains dammarane saponins similar to ginseng, it is also known as "Southern ginseng" and "Second ginseng". [2][5-7] , in addition, Gynostemma pentaphyllum also contains polysaccharides...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61P35/00A61P35/02A61K36/424C07J17/00
Inventor 朴香兰
Owner MINZU UNIVERSITY OF CHINA
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