Method for pretreating excess sludge by adopting bio-augmentation technology
A technology of excess sludge and biological enhancement, applied in biological sludge treatment, sludge treatment and other directions, can solve problems such as slow hydrolysis speed, and achieve the effect of good effect, simple operation and low energy consumption
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specific Embodiment approach 1
[0015] Specific implementation mode 1: The method for biologically enhanced pretreatment of excess sludge in this implementation mode is realized according to the following steps:
[0016] 1. Inoculate a ring of frozen-preserved Bacillus subtilis in beef extract-peptone liquid medium, culture on a rotary shaker at 30-40°C and 150r / min for 28-35 hours with ventilation, and obtain activated Bacillus subtilis;
[0017] 2. Take one ring of activated Bacillus subtilis and inoculate it in beef extract peptone solid medium containing starch, and cultivate it at 30-40°C for 30 hours to obtain Bacillus subtilis A;
[0018] 3. Add 0.2 parts of Bacillus subtilis A to 1 part of the residual sludge produced by the secondary biological treatment of sewage according to the ratio of parts by mass, and react at 30-45°C and a stirring rate of 100-150r / min for 30~ 40h, the biointensive pretreatment of excess sludge is completed;
[0019] The Bacillus subtilis in step 1 is Bacillus subtilis 1.14...
specific Embodiment approach 2
[0024] Embodiment 2: This embodiment is different from Embodiment 1 in that in Step 1, the activated Bacillus subtilis is obtained by culturing on a rotary shaker at 35° C. and 150 r / min for 30 h with aeration. Other steps and parameters are the same as those in Embodiment 1.
specific Embodiment approach 3
[0025] Embodiment 3: This embodiment differs from Embodiment 1 or Embodiment 2 in that in step 2, culture at 35° C. for 30 h to obtain Bacillus subtilis A. Other steps and parameters are the same as those in Embodiment 1 or Embodiment 2.
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