Ionizing radiation degradation method for sea cucumber polysaccharide

The technology of sea cucumber polysaccharide and ionizing radiation is applied in the field of degradation method of polysaccharide compounds, which can solve the problems of detachment of sulfate group and branched-chain sulfated fucose, cumbersome operation of oxidative decomposition method, difficult process control, etc., so as to reduce the amount of products. Loss, product molecular weight uniformity, no environmental pollution effect

Active Publication Date: 2012-07-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Previously, Yutaka Kariya (JBiochem 2002, 132, (2), 335-43) and Paulo Mourao (J Biol Chem 1996, 271, (39), 23973-84) and other research groups used dilute acid method to prepare oligosaccharides. , easily lead to the shedding of sulfate groups and branched fucose sulfates, affecting related activities
However, the oxidative decomposition method is cumbersome to operate, the process is difficult to control, and the reproducibility is poor.

Method used

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  • Ionizing radiation degradation method for sea cucumber polysaccharide
  • Ionizing radiation degradation method for sea cucumber polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: the ionizing radiation degradation method of sea cucumber polysaccharide:

[0023] Four portions of Philippine sea cucumber chondroitin sulfate (50 mg each) were weighed and prepared into aqueous solutions respectively, and the mass percent concentrations were: 1%, 2%, 5%, and 10%. All were treated with ionizing radiation, the absorbed dose was 50kGy, and the radiation dose rate was 45kGy / min; after irradiating, stand still for 1.5 hours, the non-degradable products settled to the bottom of the container, and the supernatant was taken.

[0024] The above four supernatants were vacuum freeze-dried (-40° C.) to obtain 42 mg, 39 mg, 40 mg, and 35 mg of low-molecular-weight chondroitin sulfate (ie, low-molecular-weight oligosaccharides), respectively.

[0025] HPLC high performance gel permeation chromatography (TSK3000, 4000) was used to determine the relative molecular weight, number average molecular weight, weight average molecular weight and molecular wei...

Embodiment 2

[0029] Embodiment 2: the ionizing radiation degradation method of sea cucumber polysaccharide:

[0030] Weigh 5 parts of Philippine sea cucumber chondroitin sulfate (each part is 50 mg), and prepare an aqueous solution with a concentration of 1% by mass. Treat with 5 different doses of ionizing radiation, the absorbed doses are 10KGy, 20KGy, 50KGy, 100KGy, 150KGy, and the radiation dose rate is 45kGy / min; Serum.

[0031] The above 5 supernatants were vacuum freeze-dried (-40° C.) to obtain 39 mg, 38 mg, 42 mg, 41 mg, and 37 mg of low-molecular-weight chondroitin sulfate (ie, low-molecular-weight oligosaccharides), respectively.

[0032] The relative molecular weight of degraded chondroitin sulfate at the same concentration under different irradiation doses was determined by HPLC high performance gel permeation chromatography (TSK3000). The details are shown in Table 2.

[0033] Table 2

[0034] Radiation dose (KGy)

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Abstract

The invention discloses an ionizing radiation degradation method for sea cucumber polysaccharide. The method sequentially comprises the following steps of: 1) completely dissolving the sea cucumber polysaccharide into distilled water to obtain an aqueous solution of the sea cucumber polysaccharide; 2) performing ionizing radiation degradation on the aqueous solution of the sea cucumber polysaccharide at normal temperature by adopting 60 Co, wherein the absorbed dose is 2 to 200kGy; and 3) standing and settling or centrifuging the obtained product in the 2) to remove non-degradable settlement and obtain a clear solution, drying the clear solution, and thus obtaining an oligosaccharide with low molecular weight. The product obtained by adopting the method has uniform and centralized molecular weight, the molecular weight distribution index is close to 1, and the product has good uniformity.

Description

technical field [0001] The invention relates to a method for degrading polysaccharide compounds, in particular to a method for degrading sea cucumber polysaccharides by ionizing radiation. Background technique [0002] Sea cucumber polysaccharides mainly include sea cucumber chondroitin sulfate (SC-CHS) (J Biol Chem 1996, 271, (39), 23973-84; J Biol Chem 1988, 263, (34), 18176-83; Journal of Biological Chemistry 1991, 266, (21), 13530-13536) and sea cucumber fucoidan sulfate (SC-FUC) (Carbohydr Res 1994, 255, 225-40) two polysaccharides, wherein SC-CHS is derived from sea cucumber A polysaccharide extracted from the body wall can be derived from a variety of sea cucumbers. Although their chemical structures are different, their biological activities are similar. Yutaka Kariya (J Biochem 2002, 132, (2), 335-43) extracted SC-CHS from sea cucumber, and the sugar composition analysis showed that fucose (Fuc): acetylgalactosamine (GalNAc): glucose Alkyd acid (GlcA): Sulfate (SO...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 陈士国吴念刘东红胡亚芹陈建初孙玉敬吴丹叶兴乾
Owner ZHEJIANG UNIV
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