Preparation method for extracting chitosan from hermetia illucens

A technology of black soldier fly and chitosan, which is applied in the field of preparation of chitosan extracted from black soldier fly, can solve the problems of high production cost, impact on chitosan output and quality, unstable raw materials, etc., and achieve High extraction rate, easy product quality, and the effect of controlling product quality

Active Publication Date: 2012-07-18
深圳市腾浪再生资源发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Chitosan is usually extracted from shrimp and crab shells, but the yield and quality of chitosan are affected due to the instability of raw materials, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Soak 100 g of black soldier fly pupal shells in 600 mL of 5% (mass fraction) hydrochloric acid at room temperature (26°C) for 5 hours, wash until neutral, and dry in a 60°C drying oven for 6 hours; then use 8% (mass fraction) of Soak NaOH solution in a 70°C water bath for 4h, wash until neutral, and dry in a 60°C drying oven for 6h; weigh 2g of the dried sample and soak it in 10mL of 3.6% (mass fraction) HCl solution for 0.5h, then add 40mL NaClO solution (available chlorine mole fraction: 1.0%) was treated in a water bath at 80°C for 4 hours, dried to obtain a chitin sample, and stored in a refrigerator for use. The experiment was repeated three times.

[0017] The extracted chitin product is white in color, and the average extraction rate of chitin in black soldier fly pupal shell is 13.0%. Add 1 drop of 0.03% (mass fraction) iodine-potassium iodide solution and 1 drop of 1% (mass fraction) sulfuric acid to the extracted chitin, and it will appear purple-brown on the...

Embodiment 2

[0020] Soak 100 g of black soldier fly pupal shells in 600 mL of 4% (mass fraction) hydrochloric acid at room temperature (26°C) for 12 hours, wash to neutrality, and dry in a 60°C drying oven for 6 hours; then use 6% (mass fraction) of Soak NaOH solution in a water bath at 80°C for 5h, wash until neutral, and dry in a drying oven at 60°C for 6h; weigh 4g of the dried sample and soak it in 20mL 4% (mass fraction) HCl solution for 1h, then add 80mL NaClO The solution (available chlorine mole fraction: 2.0%) was treated in a water bath at 80°C for 3 hours, dried to obtain a chitin sample, and stored in a refrigerator for use. The experiment was repeated three times.

[0021] The extracted chitin product is white in color, and the average extraction rate of chitin in black soldier fly pupal shell is 11.7%. Add 1 drop of 0.03% (mass fraction) iodine-potassium iodide solution and 1 drop of 1% (mass fraction) sulfuric acid to the extracted chitin, and it will appear purple-brown on...

Embodiment 3

[0024] Soak 100 g of black soldier fly pupal shells in 600 mL of 1% (mass fraction) hydrochloric acid (40°C) for 24 hours, wash until neutral, then dry in a 60°C drying oven for 6 hours; then use 1% (mass fraction) NaOH solution Soak in a water bath at 100°C for 24h, wash until neutral, and bake in a drying oven at 60°C for 6h; weigh 4g of the dried sample and soak it in 20mL 4% (mass fraction) HCl solution for 1h, then add 80mL NaClO solution ( Available chlorine mole fraction 5.0%) was treated in a water bath at 60° C. for 1 hour, and dried to obtain a chitin sample, which was stored in a refrigerator for use. The experiment was repeated three times.

[0025] The color of the extracted chitin product is light yellow, and the average extraction rate of chitin in black soldier fly pupal shell is 10.5%. Add 1 drop of 0.03% (mass fraction) iodine-potassium iodide solution and 1 drop of 1% (mass fraction) sulfuric acid to the extracted chitin, and it will appear purple-brown on ...

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Abstract

The invention discloses a preparation method for extracting chitosan from hermetia illucens, which comprises the following steps of: extracting chitinous substance from hermetia illucens by steps of decalcification by using hydrochloric acid, protein removal by using dilute alkalis, depigmentation by using sodium hypochlorite and the like, and removing acetyl from chitinous substance so as to obtain chitosan. The preparation method for extracting chitosan from hermetia illucens is a chitosan preparation method designed according to properties of hermetia illucens; since hermetia illucens is taken as the raw material and the sources of hermetia illucens are wide, the source channel of chitosan resources is widened; the method is simple in process, the extracting efficiency of the chitosan is high, and the quality of products is easy to control; and the chitosan prepared by the method can be applied to fields of environment protection, medical treatment, foods, textiles and agriculture.

Description

Technical field: [0001] The invention relates to a preparation method of chitosan, in particular to a preparation method of extracting chitosan from black soldier flies. Background technique: [0002] Black water (Hermtia illucens L.), English name Black soldier fly, is a kind of insect of Diptera (Diptera) Soldier Fly family. The growth and development of the black soldier fly is divided into egg stage, larval stage, pupa stage and adult stage, and the larval stage is divided into 6 different instars. The sixth instar larva is called prepupa, and the head of the larva in this stage is more mature. It is generally believed that larvae at this age no longer feed. Black soldier flies can obtain a large number of cheap products through large-scale breeding. Black soldier fly larvae, pupae and pupal shells are rich in chitin, which is a natural biological resource with high application value. [0003] Chitin is composed of N-acetyl-D-glucosamine connected by β-(1-4) glycosidi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
Inventor 徐齐云安新城韩诗畴戴建青叶静文
Owner 深圳市腾浪再生资源发展有限公司
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