Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for producing sodium alginate lyase by utilizing vibrio vulnificus

A technology for producing algin and marine vibrio by Vibrio, which can be applied in the field of bioengineering and can solve problems such as low enzyme production

Active Publication Date: 2012-07-18
EAST CHINA UNIV OF SCI & TECH
View PDF3 Cites 23 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzyme production of the original strain is low under the existing culture conditions, and there are few reports on the fermentation research of the original strain producing alginate lyase at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing sodium alginate lyase by utilizing vibrio vulnificus
  • Method for producing sodium alginate lyase by utilizing vibrio vulnificus
  • Method for producing sodium alginate lyase by utilizing vibrio vulnificus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] Embodiment 1, the fed-batch fermentation of adding 3g of solid alginate every 3h during the fermentation process

[0060] 1. Bioreactor

[0061] The fermenter used in the following examples is the FMG-5L fermenter produced by Shanghai Guoqiang Biochemical Engineering Equipment Co., Ltd.

[0062] 2. Culture medium preparation

[0063] Weigh 15g of soluble starch, gelatinize with part of deionized water; weigh 14.1g of peptone, 6g of yeast extract, 5.1g of potassium dihydrogen phosphate, 0.3g of magnesium sulfate heptahydrate, 126g of sodium chloride, and use part of deionized water Dissolve; mix the two to 3L; adjust the pH of the fermentation broth to 5.0 with hydrochloric acid after filling the tank.

[0064] Weigh 18g of solid alginate and divide it into 6 portions, put 3g in each portion into a 50ml centrifuge tube; sterilize.

[0065] 3. Fermentation culture

[0066] Marine Vibrio sp.QY102 was provided by Ocean University of China.

[0067] Pick a single colony...

Embodiment 2

[0069] Example 2, fed-batch fermentation in which 1 g of solid alginate is added every 3 hours during the fermentation process

[0070] 1. Bioreactor

[0071] Same as "1" in Example 1.

[0072] 2. Culture medium preparation

[0073] The medium preparation is the same as "2" in Example 1.

[0074] Weigh 18g of solid alginate and divide it into 18 portions, put 1g of each portion into a 50ml centrifuge tube; sterilize.

[0075] 3. Fermentation culture

[0076] Same as "3" in Example 1. The difference is that: starting from the first addition of solid alginate, add it every 3 hours, add 1g each time, and add 18 times of 18g alginate in total.

[0077] Under this process, the fermentation ended in 61 hours, and the highest enzyme activity was 36.74U / ml in 49 hours during the fermentation process. For the fermentation process curve, see figure 2 .

Embodiment 3

[0078] Example 3, fed-batch fermentation in which 3 g of solid alginate is added every 3 hours to the end of fermentation during the fermentation process

[0079] 1. Bioreactor

[0080] Same as "1" in Example 1.

[0081] 2. Culture medium preparation

[0082] The medium preparation is the same as "2" in Example 1.

[0083] Weigh several parts of solid alginate, put each part of 3g into a centrifuge tube; sterilize.

[0084] 3. Fermentation culture

[0085] Same as "3" in Example 1.

[0086] From the first addition of solid alginate, add 3g every 3h until the end of fermentation. Under this process, the fermentation ended in 61 hours, and the highest enzyme activity was 52.76U / ml in 33 hours during the fermentation process. For the fermentation process curve, see image 3 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for producing sodium alginate lyase by utilizing vibrio vulnificus, provides a novel culture medium which is suitable for fermenting and preparing high yield sodium alginate lyase and designed according to sodium alginate lyase production strains, and further provides a new method for preparing high yield sodium alginate lyase by utilizing the vibrio vulnificus is provided.

Description

technical field [0001] The invention belongs to the field of bioengineering, and relates to a microbial culture medium and a microbial fermentation process; more specifically, the invention relates to a method for producing alginate lyase by using marine vibrio. Background technique [0002] In recent years, with the vigorous development of marine drugs, the research on seaweed polysaccharides has been paid more and more attention, and alginate is one of them. Alginate, also known as sodium alginate, is a natural high-molecular polysaccharide polymer extracted from kelp, sargassum, and macroalgae. It is mainly composed of two uronic acid monomers. Alginate has a wide range of applications, and alginate degradation products have strong biological activity. Biodegradation represented by enzymatic degradation of alginate has become a trend to replace traditional chemical degradation. Alginate lyase catalyzes the degradation of alginate through the β-elimination mechanism to fo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/88C12N1/20C12R1/63
Inventor 蔡孟浩周久顺周祥山张元兴
Owner EAST CHINA UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com