All-fiber pre-amplification digital holography three-dimensional microscopic observation device

Abstract

The invention discloses an all-fiber pre-amplification digital holography three-dimensional microscopic observation device, which comprises a light source, a light splitting coupling unit, a lighting unit, a microscope objective, a curvature registration lens, a depolarization beam splitter prism, a complementary metal oxide semiconductor (CMOS) camera and a light output unit; wherein the light source is used for producing laser with a centre wavelength of 532 nanometers; the laser penetrates through the light splitting coupling unit and forms two beams of optical fiber light with the centre wavelength of 532 nanometers, and then the optical fiber light can be output to the lighting unit and the light output unit respectively; one of the two beams of optical fiber light forms into a parallel light after penetrating the lighting unit, under the irradiation of the parallel light, with information of the object appearance, object light transmitted by an object to be observed can be sent into the depolarization beam splitter prism after passing through the microscope objective and the curvature registration lens; the other beam of optical fiber light forms a spherical surface reference light after passing through the light output unit and then can be sent into the depolarization beam splitter prism; the spherical surface reference light and the object light with information of the object appearance are subjected to a light combining by the depolarization beam splitter prism, and a combined light beam is obtained, an interfered hologram formed by the combined light beam is acquired by a photo surface of the CMOS camera.

Description

technical field [0001] The invention belongs to the technical field of digital holographic microscopic imaging, and relates to a three-dimensional microscopic observation system, more particularly, to an all-fiber pre-amplification digital holographic three-dimensional microscopic observation system. Background technique [0002] High-resolution three-dimensional microscopic imaging is a very necessary observation method in microelectronics technology, precision processing technology and modern biotechnology, and plays a key role in research, development and manufacturing. Digital holography technology is the use of digital image technology, the spectrum formed by the interference of the object light wave and the reference light wave, using CCD (Charge Coupled Device, charge coupled device), CMOS (Complementary Metal-Oxide Semiconductor, metal oxide semiconductor) and other photoelectric imaging detection devices As a recording medium and recorded in a digital way, the techn...

AI Technical Summary

Problems solved by technology

[0011] (1) The pixel size of the current photoelectric image sensor is relatively large (about 3.5-10 μm), so it can only record the object light with a small angle (about less than 1°) with the reference light;

[0012] (2) The area of ​​the photosensitive surface of the photoelectric image sensor is small (about 1cm×1cm), so that the high-frequency components of the object light field cannot be recorded

[0013] Therefore, due to the above factors, serious resolution is limited, and digital holography is limited in microscopic applications.

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