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Method for measuring development stage, growth state, environment response and life span of plant

A development stage, plant growth technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of geographical environment influence, apparent observation lag, unpredictable plant state, etc., and achieve the effect of high sensitivity

Inactive Publication Date: 2014-08-13
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Apparent observation is relatively lagging, greatly affected by individual differences, affected by geographical environment, the measurement accuracy is not enough, and the state of plants cannot be predicted
At the same time, although the existing knowledge can detect the life state and age of plants from some apparent data (such as annual rings, etc.), it cannot predict the lifespan of plants.
In the prior art, there is no method to measure the developmental stage, growth state, environmental stress response and predict the lifespan of plants that links the carbon chain length of cell membrane lipids with the process of plant growth and development and environmental response, and with the lifespan of plants. Molecular Methods Reporting

Method used

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  • Method for measuring development stage, growth state, environment response and life span of plant
  • Method for measuring development stage, growth state, environment response and life span of plant

Examples

Experimental program
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Effect test

Embodiment 1

[0020] Plant membrane lipid extraction detection method comprises the following steps among the present invention: (1) get Arabidopsis thaliana (Arabidopsis thaliana) or mustard mustard (Crucihimalaya himalaica) tissue (whole plant or root or leaf or fruit pod), put into immediately Treat in 3ml of 75°C (preheated) isopropanol (containing 0.01% BHT) for 15 minutes; (2) After the sample is cooled, add 1.5ml of chloroform and 0.6ml of water, place it on a shaker, and extract at room temperature for 1 hour. After the extraction, the extract is transferred to an empty tube; then add 4ml of chloroform:methanol=2:1 (containing 0.01% BHT) to the sample and shake overnight until the leaves are whitish (when extracting with leaves, other tissues such as whole (3) Combine the obtained extracts, add 1ml of 1M KCl, shake and mix, and discard the upper layer after standing; (4) Add 2ml of distilled water to the extract , shake and mix, and discard the upper layer after standing still; (5) ...

Embodiment 2

[0025] The method for extracting and measuring membrane lipids used in this example and the method for calculating the average carbon chain length of phosphatidylserine are the same as those in Example 1. Arabidopsis seedlings grown for 20 days were irradiated with 260Gy gamma rays 1, 5, 9, and 20 days, and the carbon chain length of phosphatidylserine was detected respectively. Such as Figure 4 , the average carbon chain of Arabidopsis gradually increased at different times after gamma ray irradiation. In the present invention, the petiole of the isolated leaf of Arabidopsis is placed in deionized water to simulate the senescence of the plant leaf. Similarly, as Figure 5 , the present invention finds that during the accelerated aging process induced by plant hormones, the carbon chain length of phosphatidylserine in the leaves increases with the aging time, and hormone treatment accelerates the carbon chain increase speed.

[0026] According to this, it can be seen that i...

Embodiment 3

[0028] The membrane lipid extraction and determination method used in this example and the calculation method of the average carbon chain length of phosphatidylserine are the same as in Example 1. Arabidopsis thaliana seedlings grown for 20 days were heat-shocked at 44°C for 2 hours, and the carbon chain lengths of phosphatidylserine were detected in the control and heat-shocked plants, respectively. In the dehydration treatment, Arabidopsis leaves grown for 3 days were cut from the petiole and placed in a glass petri dish for natural dehydration. After 100 minutes, the carbon chain length of phosphatidylserine in the control and dehydration treatment was detected. As shown in Table 1, it can be seen that after heat shock treatment, the carbon chain length of phosphatidylserine in Arabidopsis leaves increased by 0.67, while dehydration treatment resulted in an increase of 0.2.

[0029] It can be seen from this that the average carbon chain of phosphatidylserine tends to increa...

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Abstract

The invention provides a molecular method for measuring development stage, growth state, and environment pressure response of a plant and predicting life span of the plant. The method comprises the step of contacting a carbon chain length of cell membrane lipid with a plant growth and development process and environment response of the plant as well as life span of the plant. The molecular method disclosed by the invention can be applied to molecular measurement of the growth and development stage, growth state, environment response and life span of the plant.

Description

technical field [0001] The invention relates to the technical field of plants, in particular to a molecular method for measuring plant developmental stages, growth states, environmental responses and predicting plant lifespan by using the chemical structure length of a basic biomolecule of plant cells. It is a method of measuring the physiological state of plants with endogenous plant microscales. Background technique [0002] Measuring the developmental stage, growth state, environmental response and predicting plant life of plants is of great importance to the breeding and cultivation of crops, the cultivation and breeding of horticultural plants, the evaluation and utilization of plant resources, and the assessment and prediction of ecosystem changes. function and potential utility value. However, the existing determination of plant growth and development status mainly depends on the observation of appearance, including morphological changes, changes in leaf color, indiv...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/00
Inventor 李唯奇郑国伟李艳贾艳霞禹晓梅张旭东郁步竹王丹丹郑艳玲
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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