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Anti-tumor saponin in anemone plants and preparation method thereof as well as application

Anemone genus, anti-tumor technology, applied in the field of medicine, to achieve the effect of high yield

Active Publication Date: 2012-07-25
SHENYANG PHARMA UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no effective method and drug to cure it. Therefore, it has become a hot spot to find a safe anti-cancer drug with less toxic and side effects through the development of natural products.

Method used

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  • Anti-tumor saponin in anemone plants and preparation method thereof as well as application
  • Anti-tumor saponin in anemone plants and preparation method thereof as well as application
  • Anti-tumor saponin in anemone plants and preparation method thereof as well as application

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0030] Pulverize 500g of anemone rhizome, pass through a 10-mesh sieve, add 8 times the amount of 0.2M 65% ethanol sodium hydroxide alkali solution, soak for 6 hours, reflux and extract three times (8 times, 8 times, 8 times the amount of alcohol added each time) ), 1.5 hours each time, combined the extraction solution, recovered until there was no alcohol smell, diluted the medicinal solution with distilled water, and adsorbed it with HPD-400 macroporous resin for 5 hours. The amount of resin used was 1kg. Elution removes impurities, and then elute with 12L 80% ethanol at a rate of 0.8L / h, collect the eluted part of 80% ethanol, spin dry, put on a silica gel column, and wash with ethyl acetate:methanol:water=65:30:8 The elution process of this step was monitored by TLC. The AR17 fraction was collected, spin-dried, and recrystallized with ethanol:methanol=6:1. The resulting product AR17 was 4.7g with a purity of 95.6%.

preparation example 2

[0032]Pulverize 500g of Anemone anemone medicinal material, pass through a 10-mesh sieve, add 6 times the amount of 0.3M 50% ethanol sodium hydroxide alkali solution, soak for 10 hours, reflux extraction three times (6 times, 6 times, 6 times), each time 2h , combine the extraction solutions, neutralize them with 2% hydrochloric acid, recover until there is no alcohol smell, dilute the medicinal solution with distilled water appropriately, and use D101 macroporous resin for adsorption for 3 hours. The amount of resin used is 2kg. h speed elution to remove impurities, washed until colorless, then eluted with 12L 80% ethanol at a speed of 0.5L / h, collected the eluted part of 80% ethanol, spin-dried, put on a silica gel column, and used ethyl acetate:methanol:water =70:30:7 elution, the elution process of this step was monitored by TLC, the AR17 fraction was collected, spin-dried, and recrystallized with ethanol:methanol=3:1, and the obtained product AR17 was 6.0 g with a purity o...

preparation example 3

[0034] Pulverize 500g of black mercury rhizomes, pass through a 16-mesh sieve, add 5 times the amount of 80% ethanol solution, soak for 12 hours, reflux and extract three times (5 times, 5 times, 5 times), each time for 1h, combine the extraction solutions, and recover to no Alcoholic taste, add NaOH to make 1.5M NaOH extract, heat at 80°C for 3h, and keep stirring. Dilute the medicinal solution with distilled water, and absorb it with HPD-300 macroporous resin for 8 hours. The amount of resin used is 1.5kg. Elution at a speed of 80% ethanol was collected, spin-dried, put on a silica gel column, eluted with ethyl acetate: methanol: water = 6: 0.5: 1, collected the eluted part, and the obtained product AR17 was 5.8 g, with a purity of was 91.6%.

[0035] Preparation Example 4 of Saponin AR17

[0036] Pulverize 500g of Anemone anemone medicinal material, pass through a 10-mesh sieve, add 10 times the amount of 0.5M KOH aqueous solution, soak for 10 hours, heat and extract thre...

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Abstract

The invention relates to a preparation method of a triterpenoid saponin compound in anemone plants and a mechanical application, in particular relates to an anti-tumor application. The molecular formula of the compound is C47H76O16, and a chemical name of the compound is oleanolic acid-3-0-alpha-L-rhamanopyranosyl-(1->2)-[beta-D-glucopyranosyl-(1->4)]-alpha-L-arabinopyranosid. The extraction preparation process of the compound disclosed by the invention is applicable to the industrial production and can be used for converting other saponin compounds in medicinal materials into the saponin through an alkaline hydrolysis production, so that the content of the target saponin compound is greatly improved. Shown by anti-tumor experiments in vivo and vitro, the compound plays a role in suppressing a plurality of kinds of tumor cells to generate, and approximately has no suppression and toxic actions on generation of normal cells. Therefore, the triterpenoid saponin compound can be used for treating tumor diseases.

Description

technical field [0001] The invention relates to the technical field of medicine, and relates to a compound with antitumor activity obtained by extracting and isolating plants of the genus Anemone and its application in antitumor. Background technique [0002] Cancer is a common disease with increasing morbidity and mortality every year. However, there is no effective method and drug to cure it. Therefore, it has become a hot spot to find a safe anti-cancer drug with low toxicity and side effects through the development of natural products. [0003] Anemone is the largest genus in Ranunculaceae (Ranunculaceae), with a total of more than 150 species of plants distributed all over the world. There are more than 50 species of anemone plants in my country, which are distributed in all provinces except Hainan Island. Anemones have a long history of medicinal use in my country, and there are many types of medicinal plants of this genus among the people, with a wide range of effec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P35/00
Inventor 路金才贾凌云王晶吕重宁林杨薛娇
Owner SHENYANG PHARMA UNIVERSITY
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