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Technique for producing alpha-linoleic acid, silkworm pupa protein and silkworm pupa peptides with silkworm pupas

A technology of silkworm chrysalis protein and linolenic acid, which is applied to the extraction of silkworm chrysalis α-linolenic acid and silkworm chrysalis protein, and the preparation of silkworm chrysalis protein hydrolyzate, which can solve the problems affecting the efficacy and low content of α-linolenic acid, and achieve the process of easy mastering , High extraction rate, light color effect

Inactive Publication Date: 2012-07-25
南宁创新科技医药技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Domestic silkworm chrysalis α-linolenic acid products generally have the disadvantage of low content, which affects the efficacy of α-linolenic acid. Therefore, domestic research focuses on increasing the content of α-linolenic acid

Method used

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  • Technique for producing alpha-linoleic acid, silkworm pupa protein and silkworm pupa peptides with silkworm pupas
  • Technique for producing alpha-linoleic acid, silkworm pupa protein and silkworm pupa peptides with silkworm pupas
  • Technique for producing alpha-linoleic acid, silkworm pupa protein and silkworm pupa peptides with silkworm pupas

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Get the dried silkworm chrysalis, remove visible sundries therein, pulverize with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of silkworm chrysalis powder in a stainless steel extraction tank, add 60 parts by weight of petroleum ether, heat up rapidly, control the temperature at 65-70° C., and stir for 2 hours. After the extraction was completed, the filtrate was obtained by filtration, the extraction step was repeated once, and the filtrates were combined twice. The solvent of the filtrate was recovered in a solvent recovery device to obtain silkworm chrysalis crude oil; the filter residue was dried in an oven at 50°C for later use. Put the silkworm chrysalis crude oil into the separation tank, add 2 times the amount of 0.1mol / L sodium hydroxide solution, stir at room temperature for 2 hours, let stand until the oil and water are separated, and release the dilute alkali solution from the bottom of the separation tank to obtain refined silkwo...

Embodiment 2

[0038] Take fresh silkworm chrysalis, dry them in an oven at 80°C, remove visible debris, crush them with a pulverizer, and pass through a 40-mesh sieve. Get 10 parts by weight of silkworm chrysalis powder in a stainless steel extraction tank, add 60 parts by weight of an extraction solvent (petroleum ether: acetone=1:1), heat up rapidly, control the temperature at 65-70°C, and stir for 2 hours. After the extraction was completed, the filtrate was obtained by filtration, the extraction step was repeated once, and the filtrates were combined twice. The solvent of the filtrate was recovered in a solvent recovery device to obtain silkworm chrysalis crude oil; the filter residue was dried in an oven at 50°C for later use. Put the silkworm chrysalis crude oil into the separation tank, add 2 times the amount of 0.1mol / L sodium carbonate solution, stir at room temperature for 2 hours, let stand until the oil and water are separated, and release the dilute alkali solution from the bot...

Embodiment 3

[0045] Take the silkworm chrysalis after reeling, dry them in an oven at 80°C, remove visible debris, crush them with a pulverizer, and pass through a 40-mesh sieve. Take 10 parts by weight of silkworm chrysalis powder in a stainless steel extraction tank, add 60 parts by weight of an extraction solvent (acetone:ethanol=1:1), heat up rapidly, control the temperature at 65-70° C., and stir for 2 hours. After the extraction was completed, the filtrate was obtained by filtration, the extraction step was repeated once, and the filtrates were combined twice. The solvent of the filtrate was recovered in a solvent recovery device to obtain silkworm chrysalis crude oil; the filter residue was dried in an oven at 50°C for later use. Put the silkworm chrysalis crude oil into the separation tank, add 2 times the amount of 0.1mol / L sodium carbonate-sodium hydroxide solution, stir at room temperature for 2 hours, let it stand until the oil and water are separated, and release the dilute al...

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Abstract

The invention discloses a method for comprehensive utilization of silkworm pupas, which includes: using fresh silkworm pupas, dry silkworm pupas, post-reeling pupas as materials, using organic solvent to extract crude silkworm pupa oil, extracting silkworm pupa alpha-linoleic acid with purity more than 85% by technical combination of carbamide embedding and molecular distillation after refining, preparing silkworm pupa protein with degreased silkworm pupa powder, and further preparing water-soluble silkworm pupa peptides from the degreased silkworm pupa powder. The preparation technique is simple in process, high in utilization rate of materials and energy sources, low in production cost and suitable for large-scale production. The silkworm pupa peptides can meet the requirements of the industries of cosmetics, foods, health-care products, medicines and the like, and have broad application prospect.

Description

technical field [0001] The invention belongs to the field of extracting active ingredients from animals, and relates to a method for extracting silkworm chrysalis α-linolenic acid and silkworm chrysalis protein and a preparation method for silkworm chrysalis protein hydrolyzate. technical background [0002] α-Linolenic acid (Linolenic acid), the scientific name is all cis-9,12,15-octadecatrienoic acid (cis9,12,15-octadecatrienoic acid), the molecular formula is C 18 h 30 o 2 , is an essential fatty acid to maintain human health. α-linolenic acid participates in the synthesis and metabolism of phospholipids in the body, and can be converted into DHA and EPA, the life-activating factors necessary for the human body. A large number of basic researches, epidemiological investigations, animal experiments, human experiments and clinical observations have shown that α-linolenic acid has various physiological functions, which is recognized by the medical and nutritional circles ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11C1/08C11C1/10C07K1/14C12P21/06A23J1/00A23J3/34
Inventor 邱英华王玉海秦志喧蒋全英
Owner 南宁创新科技医药技术有限公司
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