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Method for detecting seed purity of ricinus communist L. hybrid CSR24.181

A detection method and a technology of hybrids, which are applied in the field of detection of castor hybrid "CSR24.181" seed purity, can solve problems that have not been reported, and achieve the effects of good repeatability, stable results, and strong reliability

Inactive Publication Date: 2013-09-18
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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Problems solved by technology

At present, the method of detecting the purity of castor hybrid seeds by molecular marker technology has not been reported in the field of castor breeding

Method used

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  • Method for detecting seed purity of ricinus communist L. hybrid CSR24.181
  • Method for detecting seed purity of ricinus communist L. hybrid CSR24.181
  • Method for detecting seed purity of ricinus communist L. hybrid CSR24.181

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Embodiment Construction

[0052] The following examples further illustrate the present invention. The detection method of the embodiment of the present invention is only used to illustrate the present invention, not to limit the present invention. Simple improvements to the preparation method of the present invention under the concept of the present invention fall within the scope of the present invention.

[0053] The experimental material of this example is the hybrid seed production F of castor oil plant "CSR24.181" 1 Seeds and their parent material seeds. Cultivate for 7-10 days on a sand bed indoors at 25°C, and take the fresh young leaves.

[0054] The method of this embodiment: extract the genomic DNA of the young castor bean leaves, use SSR molecular markers for PCR amplification, subject the amplified products to gel electrophoresis, count the results and screen the characteristic primers.

[0055] 1. Extraction of castor plant genomic DNA:

[0056] Take 1-2 pieces of fresh castor-oil plant leaves th...

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Abstract

The invention discloses a method for detecting the seed purity of ricinus communist L. hybrid CSR24.181, and the method comprises the steps of: (1) extracting the genomic DNA of a seedling leaf of a ricinus communist L. hybrid seed; (2) taking the genomic DNA as a template, and selecting a specific SSR primer pair for PCR (polymerase chain reaction) amplification; (3) conducting gel electrophoresis to the amplification product; and (4) analyzing the electrophoresis result. Only a single plant having parental specific bands simultaneously can be a real CSR24.181 hybrid, and the average value of detected purity results is the seed purity of the CSR24.181 hybrid. The detection method provided in the invention has the advantages of speediness, intuitiveness, convenience, practicability, stable heredity, and insusceptibility to influences of environmental conditions, and can compensate for the shortages of traditional and conventional purity detection.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a method for detecting seed purity of castor hybrid "CSR24.181". Background technique [0002] Castor (Ricinus communist L) is an annual or perennial plant belonging to the genus Castor in the Euphorbiaceae family. It is an important industrial oil source crop and is known as a "green energy" oil crop. Because of its unique chemical properties such as low freezing point and high temperature resistance, castor oil is widely used in aviation and precision instruments as advanced lubricants, brake oils and protective oils, as well as coatings, plasticizers, nylon series, polyurethanes, fragrances, cosmetics, etc. In the production and refining of biodiesel, there are currently 175 chemical derivatives produced from castor oil and more than 2,000 series of products. The demand for castor raw materials in domestic and foreign production is on the rise, the market for raw...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 严兴初谭美莲严明芳汪磊王力军
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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