Method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop

A technology for intercellular space and tobacco leaves, which is applied in the field of measuring the intercellular space on the surface of tobacco leaves, can solve the problems of difference in appearance and quality of flue-cured tobacco, lack of quantitative evaluation, easy to be affected by subjective factors, etc., and achieves the effect of high accuracy

Inactive Publication Date: 2012-08-01
ZHENGZHOU TOBACCO RES INST OF CNTC
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Problems solved by technology

my country's tobacco planting area is vast, and the appearance quality of flue-cured tobacco in different planting areas is significantly different. In the current tobacco leaf appearance grading system, the quality factor index is largely the visual expression of the internal physiological structure and chemical composition, which is affected by the microscopic appear

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  • Method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop
  • Method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop
  • Method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop

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Embodiment Construction

[0019] Below in conjunction with specific example the inventive method is described in detail as follows:

[0020] Tobacco leaves of Lincang in Yunnan, Nanxiong in Guangdong, and Shiyan in Hubei were selected respectively, and the moisture content was balanced to 15% to 16% under constant temperature and humidity conditions. The middle 1 / 3 of the leaves were selected without variegation, disease spots, and damage. , Part of the tobacco leaf containing blue and branch veins, the leaves with a size of 1cm×1cm were cut out, and the images were observed and saved at 450 times by scanning electron microscope after the metal powder was colored. Open the image with Photoshop software, execute the window "Image" - "Histogram", read the "Pixel Value" in the pop-up menu bar, and confirm the pixel value of the entire picture is Y; use the "Magic Wand" tool to set the tolerance For 6, click the position of the cell gap in the picture, select a similar area, observe whether the selected ar...

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Abstract

A method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop is characterized that the method is a quantitative method provided for evaluation of structural indexes of the baked tobacco leaves and including: acquiring a 450-times multiplied image of a surface cell structure of initially-baked tobacco leaves by the aid of a scanning electron microscope, reading total pixel value of the image by the Photoshop software, selecting an intercellular space portion accurately by means of a magic wand, a masking, a brush, an eraser and the like, and computing surface intercellular space rate of the tobacco leaves by the ratio of pixel value of the intercellular space portion to the total pixel value of the image. The method for measuring surface intercellular spaces of the baked tobacco leaves has the advantages that by acquisition of the scanning electron microscope and processing of the Photoshop software, quantitative data of the intercellular spaces of the tobacco leaves are acquired so as to evaluate the structure of the tobacco leaves objectively. According to continuous multi-times measuring results of tobacco leaves from different production places, the method is high in accuracy, the variation coefficient of the measured results of the same sample is kept within 2.5%, and fit goodness of the evaluation results and the judgment results of specialists is high (R2=0.98).

Description

Technical field [0001] The invention involves the micro -structure of the tobacco leaves, which is specifically a technology that determines the cytoplasm gap of the surface of the tobacco leaf. It is mainly completed by scanning electron microscope analysis and Photoshop software image processing. [0002] Background technique [0003] Micro structure involves many fields such as chemistry, biology, physics, etc., refers to the structure of material and biological cells under microscope.In recent years, the application of microcosform that can be provided by scanning electron microscopy and optical microscope observing the epidermis can be provided by the epidermis of the plant organs. It has been widely used in the study of system development and quality formation as a type of valuable method.Adobe Photoshop series software has a powerful image recognition function. Nishimoto and other quantitative tests using the software to analyze the granite mode analysis of granite and Li ...

Claims

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Application Information

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IPC IPC(8): G01N23/22
Inventor 过伟民张艳玲尹启生蔡宪杰王信民刘阳王建伟张仕祥
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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