Method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop

Abstract

A method for measuring surface intercellular spaces of baked tobacco leaves by means of software Photoshop is characterized that the method is a quantitative method provided for evaluation of structural indexes of the baked tobacco leaves and including: acquiring a 450-times multiplied image of a surface cell structure of initially-baked tobacco leaves by the aid of a scanning electron microscope, reading total pixel value of the image by the Photoshop software, selecting an intercellular space portion accurately by means of a magic wand, a masking, a brush, an eraser and the like, and computing surface intercellular space rate of the tobacco leaves by the ratio of pixel value of the intercellular space portion to the total pixel value of the image. The method for measuring surface intercellular spaces of the baked tobacco leaves has the advantages that by acquisition of the scanning electron microscope and processing of the Photoshop software, quantitative data of the intercellular spaces of the tobacco leaves are acquired so as to evaluate the structure of the tobacco leaves objectively. According to continuous multi-times measuring results of tobacco leaves from different production places, the method is high in accuracy, the variation coefficient of the measured results of the same sample is kept within 2.5%, and fit goodness of the evaluation results and the judgment results of specialists is high (R2=0.98).

Description

Technical field [0001] The invention involves the micro -structure of the tobacco leaves, which is specifically a technology that determines the cytoplasm gap of the surface of the tobacco leaf. It is mainly completed by scanning electron microscope analysis and Photoshop software image processing. [0002] Background technique [0003] Micro structure involves many fields such as chemistry, biology, physics, etc., refers to the structure of material and biological cells under microscope.In recent years, the application of microcosform that can be provided by scanning electron microscopy and optical microscope observing the epidermis can be provided by the epidermis of the plant organs. It has been widely used in the study of system development and quality formation as a type of valuable method.Adobe Photoshop series software has a powerful image recognition function. Nishimoto and other quantitative tests using the software to analyze the granite mode analysis of granite and Li ...

AI Technical Summary

Problems solved by technology

my country's tobacco planting area is vast, and the appearance quality of flue-cured tobacco in different planting areas is significantly different. In the current tobacco leaf appearance grading system, the quality factor index is largely the visual expression of the internal physiological structure and chemical composition, which is affected by the microscopic appearance of the cells in the tobacco leaf. The structure has a great influence, especially the leaf structure index. Leaf structure refers to the degree of density of tobacco leaf cell arrangement (GB2635-92), which mostly depends on human subjective evaluation and is easily affected by subjective factors. At present, there is still a lack of quantitative evaluation methods

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