Method for extracting multiple alkaloids from rauvolfia vomitoria

A technology of Rauvolfia and alkaloids, which is applied in the directions of pharmaceutical formulations, plant raw materials, and medical preparations containing active ingredients, etc., can solve the problems of many extraction steps, long time consumption, low purity of yohimbine, etc., and achieve high purity Effect

Inactive Publication Date: 2012-08-08
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are reports on the simultaneous extraction of several components from Rauwolfia emetica, and there are studies on the extraction of reserpine and yohimbine, but the purity of the extracted yohimbine is low (Liu Yu, Feng Lei, Zhao Fengsheng, Pharmaceutical Biotechnology, 2008 , 15(5):393-397)
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Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 2500g of dried Rauwolfia root powder, add 50% ethanol solution containing 0.025mol / L sulfuric acid for extraction, and extract twice in total. The time is 2 hours, stirring intermittently during leaching, and filtering after each leaching.

[0032] The two extracts were combined with a volume of 31000mL, heated and concentrated under reduced pressure to 16000mL, and filtered. The filtrate was passed into 1200mL HZ-818 non-polar macroporous adsorption resin (manufactured by Huazhen Science and Technology Co., Ltd., East China University of Science and Technology) installed in the column for adsorption, and the total adsorption was 20h. Then pass 1500mL deionized water into the resin column for washing for 1.5h. Pass 2400mL of 40% ethanol solution (containing 1% hydrochloric acid) into the resin column for elution, and the elution time is 4h.

[0033]The eluate was collected, heated and concentrated under reduced pressure to 840 mL. The concentrated solution was p...

Embodiment 2

[0038] The implementation steps of emetic Rauwolfia leaching, nonpolar macroporous resin adsorption and elution, weakly acidic cation exchange resin adsorption and elution, crystallization and drying are the same as in Example 1, except that:

[0039] C 18 The eluents collected by reverse phase chromatography with higher yohimbine content were combined, with a volume of 1000mL, heated and concentrated under reduced pressure to 700mL, and then passed into a 500mL C column packed in a column. 18 Reverse phase chromatography medium for adsorption, total adsorption 2h. Then pass 2000mL 5% ethanol solution, 2000mL 10% ethanol solution, 500mL 15% ethanol solution, 500mL 20% ethanol solution, 500mL 95% ethanol solution (both containing 0.01% trifluoroacetic acid) into the column for elution, Take off time 10h. The eluate was collected in 10 fractions.

[0040] As determined by high-performance liquid chromatography, the yohimbine content in the two eluents eluted with 5% ethanol s...

Embodiment 3

[0044] Take 2500g of dried Rauwolfia root powder, add 50% ethanol solution containing 0.025mol / L sulfuric acid for extraction, and extract twice in total. The time is 2 hours, stirring intermittently during leaching, and filtering after each leaching.

[0045] The two extracts were combined to a volume of 30,000 mL, heated and concentrated under reduced pressure to 15,000 mL, and filtered. The filtrate was passed through 1200mL HZ-818 non-polar macroporous adsorption resin installed in the column for adsorption for a total of 18 hours. Then pass 1500mL deionized water into the resin column for washing for 1.5h. Pass 2500mL of 40% ethanol solution (containing 1% hydrochloric acid) into the resin column for elution, and the elution time is 4h. Then pass 3000mL of 80% ethanol solution (containing 1% hydrochloric acid) into the resin column for elution, and the elution time is 6h.

[0046] Collect the eluate from 80% ethanol solution, heat and concentrate under reduced pressure...

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PUM

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Abstract

The invention discloses a method for extracting multiple alkaloids from rauvolfia vomitoria. The method comprises the following steps of: adding acid-containing ethanol aqueous solution into root powder of the rauvolfia vomitoria, stirring and filtering to obtain leach liquor; concentrating the leach liquor, adsorbing with non-polar macroporous adsorption resin, washing and eluting with 40 percent ethanol and 80 percent ethanol; collecting eluate respectively, concentrating and then adsorbing with weakly acidic cation exchange resin, washing and eluting with 50 percent ethanol; collecting eluate respectively, adjusting pH to be 3 by using concentrated acid, concentrating and then adsorbing with a C18 reversed phase chromatography medium and eluting with the ethanol aqueous solution with gradually increasing concentration; and collecting the eluate in stages respectively, combining the parts with higher single alkaloid purity in the eluate respectively, concentrating to obtain solid, adding an organic solvent to crystallize to obtain crystals of yohimbine, ajmaline, reserpoid, reserpine and rescinnamine. According to the method, five alkaloids can be extracted from a plant at most at one time; and the method has the advantages of few steps, less dosage of the used organic solvent, low pollution and simple required equipment.

Description

technical field [0001] The present invention relates to a drug separation method in the technical field of medicine, specifically extracting yohimbine, ajmaline, and serpentine from the plant Rauwolfia vomitoria Afz. ), reserpine (reserpine), lixinamine (rescinnamine) and other five alkaloid methods. Background technique [0002] Rauwolfia is a plant of the genus Rauwolfia in the family Apocynaceae, which contains a variety of medicinal ingredients. Emetic Rauvolwolfia is a kind of Rauwolfia, which was introduced from Africa. It contains various alkaloids such as reserpine, amalline, yohimbine, and serpentine. These alkaloids can be used as medicines and have clinical effects on Therapeutic effect of many diseases. [0003] According to the literature search of the prior art, it is found that extracting medicinal components from Rauvolwolfia (including emetic Rauvolwolfia) is mostly reported to extract reserpine, which was extracted with benzene in the early stage and then...

Claims

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Application Information

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IPC IPC(8): C07D459/00A61K36/24
Inventor 赵凤生岳京丽冯蕾
Owner SHANGHAI JIAO TONG UNIV
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