Method for extracting multiple alkaloids from rauvolfia vomitoria

Abstract

The invention discloses a method for extracting multiple alkaloids from rauvolfia vomitoria. The method comprises the following steps of: adding acid-containing ethanol aqueous solution into root powder of the rauvolfia vomitoria, stirring and filtering to obtain leach liquor; concentrating the leach liquor, adsorbing with non-polar macroporous adsorption resin, washing and eluting with 40 percent ethanol and 80 percent ethanol; collecting eluate respectively, concentrating and then adsorbing with weakly acidic cation exchange resin, washing and eluting with 50 percent ethanol; collecting eluate respectively, adjusting pH to be 3 by using concentrated acid, concentrating and then adsorbing with a C18 reversed phase chromatography medium and eluting with the ethanol aqueous solution with gradually increasing concentration; and collecting the eluate in stages respectively, combining the parts with higher single alkaloid purity in the eluate respectively, concentrating to obtain solid, adding an organic solvent to crystallize to obtain crystals of yohimbine, ajmaline, reserpoid, reserpine and rescinnamine. According to the method, five alkaloids can be extracted from a plant at most at one time; and the method has the advantages of few steps, less dosage of the used organic solvent, low pollution and simple required equipment.

Description

technical field [0001] The present invention relates to a drug separation method in the technical field of medicine, specifically extracting yohimbine, ajmaline, and serpentine from the plant Rauwolfia vomitoria Afz. ), reserpine (reserpine), lixinamine (rescinnamine) and other five alkaloid methods. Background technique [0002] Rauwolfia is a plant of the genus Rauwolfia in the family Apocynaceae, which contains a variety of medicinal ingredients. Emetic Rauvolwolfia is a kind of Rauwolfia, which was introduced from Africa. It contains various alkaloids such as reserpine, amalline, yohimbine, and serpentine. These alkaloids can be used as medicines and have clinical effects on Therapeutic effect of many diseases. [0003] According to the literature search of the prior art, it is found that extracting medicinal components from Rauvolwolfia (including emetic Rauvolwolfia) is mostly reported to extract reserpine, which was extracted with benzene in the early stage and then...

AI Technical Summary

Problems solved by technology

There are reports on the simultaneous extraction of several components from Rauwolfia emetica, and there are studies on the extraction of reserpine and yohimbine, but the purity of the extracted yohimbine is low (Liu Yu, Feng Lei, Zhao Fengsheng, Pharmaceutical Biotechnology, 2008 , 15(5):393-397)

There are also reports of simultaneous extraction of reserpine, respirin and rawolfia from emetic Rauwolfia, but there are many extraction steps and it takes a long time (Zhou Xueqing, Feng Yuhong, Zhang Chong, Chen Sili, Lin Qiang, Hainan University Journal of Natural Sciences, 2008, 26(2): 145-148)