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Method for extracting darutoside from herba siegesbeckiae

A technology of Darutoside and Darutoside, which is applied in the field of extracting Darutoside, can solve the problems of long cycle time, large amount of volatile reagents used, and low yield, and achieve the effect of reducing the dosage

Inactive Publication Date: 2012-08-08
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As mentioned above, the existing method for separating Darutoside has complicated operation, low yield, large amount of volatile reagents, small production volume and long cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Grind the raw material of Ulus chinensis, take 5kg, add 10 times the amount of 70% ethanol solution to reflux and extract twice, concentrate the extract until it is alcohol-free, disperse it with an appropriate amount of water, add it to 3LX-5 macroporous resin for adsorption, wash the resin without sugar color, and then use 15L of 70% ethanol solution for elution, the eluent was concentrated under reduced pressure, the concentrated solution was extracted twice with an equal volume of ethyl acetate, the extract was passed through a neutral alumina column (120-300 mesh), and the ethyl acetate was recovered through the column liquid to obtain Extract 9g. Take n-hexane, ethyl acetate, methanol, and water and mix them according to 1:3:2:4. After fully layering, take the upper phase and fill the high-speed countercurrent chromatography column. At the same time, turn on the main engine at 800rpm, and pump the lower phase as the mobile phase. After the system is balanced, the ...

Embodiment 2

[0019] Grind the raw material of Weltia chinensis, take 5kg, add 7 times the amount of 80% ethanol solution to reflux and extract twice, concentrate the extract to no alcohol, disperse with an appropriate amount of water, add 2LHPD400 macroporous resin for adsorption, wash the resin with water without sugar color, and then use 10L of 60% ethanol solution, the eluate was concentrated under reduced pressure, the concentrated solution was extracted twice with an equal volume of ethyl acetate, the extract was passed through a short column of neutral alumina (120-300 mesh), and the ethyl acetate was recovered through the column liquid to obtain the Cream 11g. Take n-hexane, ethyl acetate, methanol, and water and mix them according to 2:5:4:5. After fully layering, take the upper phase and fill the high-speed countercurrent chromatography column. At the same time, turn on the host at 900 rpm, and pump the lower phase as the mobile phase. After the system is balanced, the flow rate i...

Embodiment 3

[0021] Grind the raw material of Ulus sativa, take 5kg, add 6 times the amount of 60% ethanol solution to reflux and extract for 3 times, concentrate the extract to no alcohol, disperse with an appropriate amount of water, add 2.5LAB-8 macroporous resin for adsorption, wash the resin without sugar color, and then Elute with 10L 80% ethanol solution, concentrate the eluent under reduced pressure, extract the concentrated solution twice with an equal volume of ethyl acetate, pass the extract through a short column of neutral alumina (120-300 mesh), and recover ethyl acetate through the column liquid , to obtain extract 9.5g. Take n-hexane, ethyl acetate, methanol, and water and mix them according to 3:8:5:9. After fully layering, take the upper phase and fill the high-speed countercurrent chromatography column. At the same time, turn on the main engine at 700rpm, and pump the lower phase as the mobile phase. After the system is balanced, the flow rate is adjusted to 2ml / min. At ...

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Abstract

The invention discloses a method for extracting darutoside from herba siegesbeckiae. The method comprises the following steps of: a, crushing the herba siegesbeckiae raw material, adding 40-90 percent ethanol solution in an amount which is 5-10 times that of the herba siegesbeckiae raw material and performing reflux extraction for two to three times, concentrating extract to be alcohol-free, adding into macroporous resin for adsorption, washing the resin until no caramel color exists, eluting with 30-80 percent ethanol solution and performing reduced pressure concentration on eluate to obtain concentrate; b, extracting the concentrate with acetic ether, flowing extract through an aluminum oxide column and recovering reagent from the column flowing liquid to obtain extractum; and c, separating the extractum by using high-speed counter-current chromatography, performing on-line monitoring through an ultraviolet detector, collecting a target fraction and performing reduced pressure drying to obtain the darutoside. The method has the advantages of simple operation, high efficiency, large preparation capacity, short period and the like and is suitable for being promoted and used.

Description

technical field [0001] The present invention relates to a method for extracting darutoside from Uxus chinensis, in particular to a method for extracting darutoside from Uxia chinensis by using macroporous resin and high-speed countercurrent chromatography. Background technique [0002] Scutellaria chinensis is the dry above-ground part of snails, stalks, or hairy stalks of Asteraceae plants. It is also called fat pigweed, fat pig vegetable, sticky cubeb, sticky vegetable, yellow flower, and sticky. According to traditional medicine, Xishui grass is cold in nature, has the functions of dispelling rheumatism, sharpening joints, and detoxifying. It is often used to treat rheumatic arthralgia, weakness of the muscles and bones, soreness of the waist and knees, paralysis of the limbs, hemiplegia, and rubella eczema. Modern medical research has found that the main components of Sisi terpenoids, sesquiterpenoids, flavonoids, etc., such as darutoside, darugenin, kynonyl alcohol, d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/24C07H1/08
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH