Method for detecting trace biomolecule ionizing radiation decomposition reaction based surface enhanced Raman spectrum

A technology of surface-enhanced Raman and biomolecules, which is applied in the interdisciplinary field of biological detection and radiation biology, and achieves good reproducible results

Active Publication Date: 2012-08-08
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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Problems solved by technology

However, so far, there have been no reports on the application of this technology in the field of radiation biology. Moreover, to use this technology to detect radiation damage to biomolecules, the key is to obtain biomolecules with good adsorption and stability. Substrates with high, repeatable measurement, high SERS activity, and whether they can withstand the damage and interference that may be caused by general energy-carrying ion radiation conditions and the environment

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  • Method for detecting trace biomolecule ionizing radiation decomposition reaction based surface enhanced Raman spectrum
  • Method for detecting trace biomolecule ionizing radiation decomposition reaction based surface enhanced Raman spectrum
  • Method for detecting trace biomolecule ionizing radiation decomposition reaction based surface enhanced Raman spectrum

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[0036] The basic guiding idea of ​​the present invention is to carry out the SERS spectrum experiment which can be positioned, can be measured repeatedly, and detects traces. This is because when carrying out irradiation experiments in which the radiation dose of biomolecules changes with an increase, it is necessary to remove the tested sample from the stage of the Raman spectroscopy microscope, and then repeat the measurement after irradiation. Here, the effective solution proposed by the present invention is to use a SERS substrate with a micro-nano structure, because the advantage of using this microsphere containing a nano-structure is that the measurement point can be easily positioned and identified with a microscope, thus taking away the sample radiation. After the photo is taken, the original measuring point can be found again for measurement. Among them, each micron sphere is dispersed, and its size should be smaller than the focused laser spot. For a schematic diag...

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Abstract

The invention discloses a method for detecting trace biomolecule ionizing radiation decomposition reaction based surface enhanced Raman spectrum (SERS). The method comprises three SERS substrates suitable for the biomolecule radiation test and the corresponding test method. The method comprises the following steps: using a micron-sized semisphere consisting of a silver nano sheet as the SERS substrate and quantitatively measuring the radiation damage of a solid biological sample (such as protein) under the direction action of ionizing radiation; using a gold wrapped silicon dioxide submicron sphere as the SERS substrate, connecting DNA and quantitatively measuring the radiation damage of the DNA molecule of a solid biological sample under the direction action of ionizing radiation; and mixing silver colloid particles and a sample from a radiation reaction solution, measuring the product of the radiation decomposition reaction by utilizing SERS effect and researching the radiation damage of the biomolecule under the indirect action of radiation. Due to the method, the radiation decomposition reaction of the trace biomolecule caused by energetic ions can be subjected to on-line, non-destructive and trace detection conveniently.

Description

technical field [0001] The invention belongs to the interdisciplinary field of biological detection and radiation biology, and relates to a method for detecting the radiolysis reaction process and products of trace biomolecules under the action of ionizing radiation, especially energy-carrying ion irradiation, by using surface-enhanced Raman spectroscopy. Background technique [0002] Due to the rapid development of nuclear technology in the fields of agriculture, industry, biology and medicine, people are increasingly concerned about the biological effects of radiation, nuclear safety protection, and the impact of radiation on plants, animals and humans. In particular, the recent 9.0-magnitude earthquake in Japan triggered a nuclear power plant explosion crisis, and nuclear radiation protection has become a major problem that needs to be solved urgently. There is an urgent need to understand the effects and effects of ionizing radiation related to nuclear radiation on livin...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 黄青柯志刚鲁逸林孟国文朱储红黄竹林余增亮
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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