Recombinant chimeric protein carrying rotavirus antigen epitope and preparation thereof

An antigenic epitope, rotavirus technology, applied in the field of medical biology, can solve the problems of viral nucleic acid pollution, can not fully protect RV infection, can only reduce mortality and severe diarrhea
CN102643348BActive Publication Date: 2014-07-02INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
INST OF MEDICAL BIOLOGY CHINESE ACAD OF MEDICAL SCI
Publication Date
2014-07-02
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Abstract

The present invention provides a recombinant chimeric protein carrying rotavirus antigenic epitopes and preparation thereof, respectively carrying rotavirus P[4]223, P[4]56, G1[142], G4[208], Six recombinant proteins with P[8]296, G2[87] epitopes, and P[4]223, P[4]56, G1[142], G4[208], P[8] 296 and G2[87] epitope recombinant protein, detected by immunological WesternBlot, said each recombinant chimeric protein can be combined with anti-rotavirus whole virus, anti-rotavirus VP6 and anti-corresponding rotavirus VP4 / VP7 antibody specific immune reaction. It shows that the above-mentioned recombinant chimeric proteins have good immunoreactivity of the VP6 carrier protein and the inserted rotavirus epitope, and are not infectious, have no side effects such as virulence reversion and intussusception, and have no effect on Further development of rotavirus RV recombinant chimeric vaccine has high application value.
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Description

technical field

[0001] The invention relates to a recombinant chimeric protein, in particular to a recombinant chimeric protein carrying a rotavirus epitope and its preparation, belonging to the field of medical biotechnology. Background technique

[0002] Rotavirus (RV) is the main pathogen of infantile diarrhea. There are more than 600,000 RV infection deaths worldwide each year, 80% of which occur in developing countries. RV belongs to Reoviridae ( Reoviridae ), rotavirus ( Rotaviruses ). The RV genome consists of 11 fragments of double-stranded RNA, encoding 6 structural proteins (VP1-VP4, VP6 and VP7) and 6 non-structural proteins (NSP1-NSP5 / NSP6), respectively.

[0003] According to the different antigenicity of the antigenic protein VP6 of the RV group (subgroup), the rotavirus RV discovered so far can be divided into seven groups A to G, and group A is the main pathogen of infantile diarrhea. The 12 proteins (genes) of group A rotavirus RV can be divided into d...

Claims

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