Method for separating, purifying and culturing human blood and marrow dendritic cells

A dendritic cell and bone marrow technology, applied in the field of biomedicine, can solve the problem of inability to efficiently expand limited dendritic cells, and achieve the effects of simple and clear operation process, low pyrogen, and shortened operation time.

Active Publication Date: 2012-08-22
天津市灏洋生物制品科技有限责任公司
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Problems solved by technology

[0003] The present invention proposes a method for separating, purifying and culturing dendritic cells from human blood and bone marrow, which solves the problem that limited dendritic cells cannot be efficiently expanded in the prior art

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  • Method for separating, purifying and culturing human blood and marrow dendritic cells
  • Method for separating, purifying and culturing human blood and marrow dendritic cells

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Embodiment Construction

[0037] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

[0038] Such as figure 1 As shown, a method for separating, purifying and culturing human blood and bone marrow dendritic cells comprises the following steps:

[0039] 1. Use hydroxyethyl starch and diatrizoate to prepare human blood and bone marrow dendritic cell separation solution. The steps are as follows:

[0040] 1.1. Weigh 40g of hydroxyethyl starch, heat and dissolve it in distilled water, and distill the volume to 100ml with double distilled water to make ...

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Abstract

The invention provides a method for separating, purifying and culturing human blood and marrow dendritic cells, which includes the following steps that: hydroxyethyl starch and meglumine diatrizoate are used for preparing human blood and marrow dendritic cell-separating solution; sodium carbonate, sodium bicarbonate and CD123 antibody are used for producing a human blood and marrow dendritic cell culture flask; L-proline, insulin, vitamin C, L-glutamine, human umbilical cord blood plasm, recombinant human fibroblast growth factor and DMEM/F12 basic medium are used for preparing human blood and marrow dendritic cell culture solution; and human blood and marrow dendritic cells are separated, purified and cultured. The method for separating, purifying and culturing human blood and marrow dendritic cells can effectively prepare a large quantity of human blood and marrow dendritic cells, and is divided into four parts, and compared with the conventional method, the method has the advantages of simple and clear operation process and shorter operation time.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a method for separating, purifying and culturing human blood and bone marrow dendritic cells. Background technique [0002] Dendritic cells (dendritic cells; DC) are one of the antigen-presenting cells (APCs) present in peripheral blood, skin, lymphoid organs and thymus, and are enriched in lymphoid and non-lymphoid tissues. Dendritic cells have a strong ability to present antigens, which can express antigenic peptides to class I and class II dendritic cells, and activate CD4 and CD8 T cells, respectively, thereby inducing in vivo response to specific antigens (pathogenic microbial antigens) , tumor antigen, transplant antigen, etc.) immune response. The powerful immune-inducing function of DC is very suitable for immunotherapy (DC treatment) of various tumors. Previous reports have shown that DCs stimulated with Sendai virus (SeV) have strong anti-tumor effects in mouse ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0784
Inventor 高旭
Owner 天津市灏洋生物制品科技有限责任公司
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