Taxus chinensis var. maire embryo in-vitro seedling forming technique and culture medium employed by same

Abstract

The invention discloses a Taxus chinensis var. maire embryo in-vitro seedling forming technique, which comprises the following steps: removing aril from Taxus chinensis var. maire seeds, disinfecting by using alcohol and sodium hypochlorite in sequence, removing hard testa, taking embryos out, cleaning the embryos in sterile water, inoculating on a specific test-tube culture medium, performing dark culture, culturing under a lamp into seedlings after the embryos are sprouted, putting test-tube plantlets in an environment at the temperature of 25DEG C, light intensity of 1,500lx, illumination of 12h/d and relative humidity (RH) of about 80 percent for 7 days to complete in-bottle seedling hardening, transplanting the test-tube plantlets to a specific medium, controlling the temperatures atday and night to 20-25DEG C and 15-18DEG C, the light intensity at noon to 25-40klx and the RH to 70-85 percent, completing out-bottle medium seedling hardening after 25 days, and transplanting the test-tube plantlets to the field. By the Taxus chinensis var. maire embryo in-vitro seedling forming technique, the Taxus chinensis var. maire embryos are sprouted to form seedlings in the same year, and a quick method is provided for quickly propagating Taxus chinensis var. maire seedlings and protecting resources.

Description

technical field [0001] The technology for in vitro seedling formation of southern yew embryos belongs to the field of biotechnology, and relates to an in vitro culture technology for southern yew embryos and a culture medium used therefor. Background technique [0002] Southern yew ( Taxus chinensis var. Maire ), which is a unique Tertiary relic tree species in my country. In the natural state, due to reasons such as unisexual flowers, dioecious plants, and cross-pollination, the number of seeds formed is small; and the seed dormancy period is long, and it takes two winters and one summer to germinate under natural conditions. The germination rate and seedling survival rate In addition, it grows slowly (it takes 60 years for a tree to mature), and its ability to grow and compete in the population is weak, resulting in less and less natural distribution. In addition, due to the special timber and medicinal value of southern yew, its resources have been devastated since the...

AI Technical Summary

Problems solved by technology

In the natural state, due to reasons such as unisexual flowers, dioecious plants, and cross-pollination, the number of seeds formed is small; and the seed dormancy period is long, and it takes two winters and one summer to germinate under natural conditions. The germination rate and seedling survival rate Low; coupled with its slow growth (it takes 60 years for a tree to mature), its growth and competitiveness in the population are weak, resulting in less and less natural distribution