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Determination method of rhodamine b content in plastic packaging materials

A technology of plastic packaging and detection methods, applied in the direction of material separation, material analysis, measuring devices, etc., to achieve the effects of accurate quantification, high detection accuracy, and easy operation

Active Publication Date: 2015-08-26
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the detection of rhodamine B in plastic packaging materials that directly contact food

Method used

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  • Determination method of rhodamine b content in plastic packaging materials
  • Determination method of rhodamine b content in plastic packaging materials
  • Determination method of rhodamine b content in plastic packaging materials

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: The steps of the detection method of Rhodamine B content in this plastic packaging material are as follows.

[0020] Break the plastic rope to a single particle ≤0.02g, accurately weigh 0.5g (accurate to 0.001g), add 50mL methanol, ultrasonically extract for 10min, ultrasonic frequency 40-80kHz, take out the supernatant, and continue to extract the bottom residue with 50mL methanol , sonicated for 10 minutes, and combined the two extracts; the extract was concentrated to nearly dryness with a rotary evaporator, blown dry with a nitrogen blower, and then fixed to 10 mL with methanol, and passed through a 0.22 μm filter membrane to obtain a sample solution; 1 μL of the sample solution was used in the United States Waters Acquity-TQD ultra-high performance liquid chromatography tandem mass spectrometry detection, the chromatographic column is a BEH reversed-phase C18 ultra-high-efficiency chromatographic column, the specification is 50mm*2.1mm, the particle si...

Embodiment 2

[0029] Embodiment 2: The steps of the detection method of rhodamine B content in this plastic packaging material are as follows.

[0030] Cut the plastic packaging bag to a single particle ≤ 0.02g, accurately weigh 0.2g (accurate to 0.001g), add 20mL of acetonitrile, vortex extract for 20min, vortex power 40-150W, take out the supernatant as the extract; extract Concentrate to near dryness with a liquid rotary evaporator, blow dry with a nitrogen blower, then dilute to 5 mL with acetonitrile, and pass through a 0.22 μm filter membrane to obtain a sample solution; 5 μL of the sample solution is detected by an American Waters Acquity-TQD ultra-high performance liquid chromatography tandem mass spectrometry , The chromatographic column is a BEH reversed-phase C18 ultra-high-efficiency chromatographic column with a specification of 50mm*2.1mm and a particle size of 1.7μm. The mobile phase is a methanol-formic acid water system, and the area external standard method is used for quan...

Embodiment 3

[0037] Embodiment 3: The steps of the detection method of rhodamine B content in this plastic packaging material are as follows.

[0038]Crush the plastic bottle, accurately weigh 0.5g (accurate to 0.001g), add the rhodamine B standard sample into the powder sample in three gradients, add 50mL of methanol, extract by mechanical vibration for 30min at a frequency of 100-200rpm, take out The supernatant and the residue in the lower layer were extracted with 20 mL of methanol, and mechanically oscillated for 20 minutes; the supernatant was taken out again, and the residue in the lower layer was extracted with 10 mL of methanol, mechanically oscillated for 10 min, and the extracts were combined three times; Blow dry, then dilute to 5mL with methanol, pass through a 0.22μm filter membrane to obtain a sample solution; take 7μL sample solution and use American Waters Acquity-TQD ultra-high performance liquid chromatography tandem mass spectrometry detection, the chromatographic column...

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Abstract

The invention discloses a method for detecting Rhodamine B content in a plastic packaging material. The method comprises the following steps of (1) crushing the plastic packaging material into plastic powder; (2) carrying out ultrasonic extraction, vortex extraction or mechanical oscillation extraction on the plastic powder with methyl alcohol or acetonitrile; concentrating until extract liquor is dry; and enabling the plastic powder to achieve a constant volume by using the same solvent, so as to obtain a sample solution; (3) carrying out high performance liquid chromatography tandem mass spectrum detection on the sample solution, wherein a chromatographic column is an inverse C18 high performance chromatographic column, and a mobile phase is an acetonitrile-methanoic acid water system or a methanoic acid-methanoic acid water system; and quantifying by using an external standard method. The method can effectively detect the Rhodamine B in the plastic packaging material by combining pretreatment with high performance liquid chromatography tandem mass spectrum technology. The method provided by the invention has the characteristics of simplicity and convenience in operation by treating the plastic packaging material through ultrasonic, vortex and mechanical oscillation technologies. With the adoption of the high performance liquid chromatography tandem mass spectrum detection, the method has the detection limit which is low to 0.1 mug / kg and has the characteristics of qualitative and quantitative accuracy and high detection precision.

Description

technical field [0001] The invention relates to a method for detecting toxic substances in plastic packaging materials, in particular to a method for detecting rhodamine B content in plastic packaging materials. Background technique [0002] Rhodamine B (Rhoda mine B), also known as rose red B, or alkaline rhodamine, commonly known as flower pink, is a kind of artificially synthesized dye with bright pink color, which was once used as a food additive, but it was later proved by mouse experiments Rhodamine B can cause subcutaneous tissue sarcoma, and the median lethal dose is 500 mg / kg. The United States has conducted research on this substance, showing that it is carcinogenic. Rhodamine B not only causes cancer, but also may cause red staining of human skin and viscera, mild congestion of blood vessels between the brain, rupture of myocardial fibers, blurred and disappearing stripes; pulmonary edema, a large amount of homogeneous red staining in the alveolar cavity; Necrosi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/89
Inventor 连运河张玉芬王磊程淑君高伟
Owner CHENGUANG BIOTECH GRP CO LTD
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