Integrated cartridge

A technology of cartridge and filter material, applied in the field of analyzing biomolecules

Inactive Publication Date: 2012-09-12
AKONNI BIOSYSTEMS INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The thermal expansion of air is more than 5 times greater

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0099] Example 1: Components of a Microarray-Based Sample Analysis (MBSA) System

[0100] A. Fluid Subsystem

[0101] The fluidic subsystem consists of three types of functional components: a bidirectional microfluidic pump, a selector valve, and a cartridge "needle" valve. Figure 5 is a schematic diagram showing the fluid subsystem of the pump and selector valve connected to the integrated cartridge. The fluid layout uses a combination of bi-directional pumps and selector valves available from Global FIA. Such as Figure 5 As shown, the fluidic subsystem is connected to the integrated cartridge by a fluid manifold 90 and holds various reagents in storage containers external to the integrated cartridge 100 .

[0102] Figure 6A One embodiment of fluid manifold 90 is shown. In this embodiment, the manifold 90 allows two fluidic fittings 91 to be connected to each cartridge port 72 . The manifold has eight bosses 94 with Luer tips. These sleeves have fluid channels that ...

Embodiment 2

[0130] Example 2: Using an integrated cartridge and a microarray-based sample analysis system to detect bacteria in a test sample.

[0131] The sequence of events for the MBSA system is as follows. A user introduces a sample into the integrated cartridge. The system then performs the following automated steps: Prepare the sample, use the BladderThermocycler TM Perform PCR, mix the PCR product with hybridization and / or APEX reaction mixture, transfer the mixture to the microarray chamber, perform hybridization with the microarray, and then detect the microarray image.

[0132] In one embodiment, the sequence of sample preparation on the cartridge is: introduce sample into sample column, introduce binding buffer into sample column, mix with air, transport sample mixture to TruTip column, switch between columns, dispense to Waste, introduce wash buffer into TruTip columns, switch between columns, distribute to waste, introduce elution buffer, then distribute to elution columns....

Embodiment 3

[0142] Example 3: Genotyping of single nucleotide polymorphisms (SNP typing) using an integrated cartridge and a microarray-based sample analysis system

[0143] This example shows the feasibility of a microfluidic controlled system for SNP typing of physical markers that can be used in forensic applications. The experimental setup combined components for sample preparation, PCR amplification, and allele-specific primer extension microarray (AS-APEX) to form an all-in-one system to detect markers for eye color. The system includes fluid handling subsystems (e.g. pumps), thermal cycling subsystems (e.g. Akonni Bladder Thermocycler), optical instruments (e.g. Akonni Reader), disposable integrated cartridges (i.e. Akonni TruTip, Akonni PCR / TruArray flow cell , microfluidic pathways and microfluidic valves) and a cartridge docking station.

[0144] The 6 SNPs listed in Table 1 were used as test models for the experiments. These SNPs have been reported in the literature as major ...

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Abstract

An integrated cartridge for sample processing and analysis is disclosed. The integrated cartridge contains a sample preparation chamber having a sample inlet and a sample outlet, and a sample purification chamber adapted to receive a replaceable sample purification unit containing a housing and an extraction filter inside the housing. The extraction filter specifically binds to a molecule of interest. The sample purification chamber has a sample inlet that is in fluid communication with the sample outlet of the sample preparation chamber. Also disclosed is a microarray-based sample analysis (MBSA) system.

Description

[0001] related application [0002] This application claims priority to US Provisional Application No. 61 / 272,397, filed September 21, 2009, which is hereby incorporated by reference in its entirety. technical field [0003] The technical field is biotechnology, more specifically methods and devices for the analysis of biomolecules. Background of the invention [0004] It is desirable to obtain an analytical instrument that combines the functions of sample preparation and sample analysis. It is also desirable to have analytical instruments that are light and small and can be produced at low cost. However, microfluidic challenges have hindered the development of such analytical devices. These challenges are partly due to fluid dynamics at small scales. For example, according to the Hagen-Poiseuille equation, as the diameter of a microfluidic channel decreases, the pressure drop across the channel increases to the power of 4. These large pressure drops can lead to very unp...

Claims

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Application Information

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IPC IPC(8): G01N35/00G01N1/34G01N1/38G01N33/48C12Q1/68B01F33/40
CPCG01N1/34G01N21/01G01N21/6486B01L3/502715B01L2200/026B01L2200/10B01L2300/0681C12Q1/6837
Inventor 菲利普·贝尔格雷德尔克里斯托弗·G·库尼艾里克山大·N·派洛夫
Owner AKONNI BIOSYSTEMS INC
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