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Denitrifying bacteria and aquatic plant-microbe combined rehabilitation method using same

A denitrifying bacteria and water body technology, applied in the field of environmental (water body) pollution control, can solve the problems of less research on the treatment of nitrogen eutrophication in water bodies, and achieve the effects of increasing survival rate, saving costs, and improving nitrogen removal rate

Inactive Publication Date: 2013-05-29
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a lot of research has been carried out on the remediation of water pollution by plants or microorganisms, but there are few studies on the treatment of nitrogen eutrophication in water bodies through the combined remediation technology of plants and microorganisms.

Method used

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  • Denitrifying bacteria and aquatic plant-microbe combined rehabilitation method using same
  • Denitrifying bacteria and aquatic plant-microbe combined rehabilitation method using same
  • Denitrifying bacteria and aquatic plant-microbe combined rehabilitation method using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1 strain screening and identification

[0029] 1.1 Strain isolation and screening

[0030] The soil samples used in the test were collected from the roots of aquatic plants along several rivers and lakes in Nanjing, the bottom sludge of sewage treatment plants, and the soil of paddy fields in Lianyungang. Vigorously growing plants are selected, and after shaking off a large piece of surface soil, the remaining part is used as plant rhizosphere soil.

[0031] Weigh 10 g of the collected plant root soil sample, put it into 90 mL of sterile water containing glass beads, shake it for 20 min, and let it stand for 20-30 s to make a bacterial suspension. The bacterial suspension was transferred to DM medium with a 10% inoculum size and cultured at 28°C. Under aseptic conditions, take 1mL of the bacterial suspension and put it into a 9mL sterile water glass tube, mix well, and then make 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 Diluents of various concentrations.

[...

Embodiment 2

[0063] Example 2 Research on the denitrification characteristics of Pseudomonas LR

[0064] 2.1 Denitrification capacity determination

[0065] Take 8 mL of the bacterial suspension cultured at 28°C for 36 hours and inoculate it into 500 mL of sterilized DM culture solution (packed in a triangular bottle), mix evenly, and then divide into sterile test tubes, 10 mL each, and culture at 28°C for 6 hours and 12 hours respectively. , 18h, 24h, 30h, 36h, 42h, 48h, 54h, 60h, 66h, and 72h, take samples, take them out and store them in a refrigerator at 4°C, take out 3 tubes each time, and measure the removal rate of nitrate nitrogen after all are taken out.

[0066] When the bacteria are inoculated into the fresh DM medium, the bacteria do not grow and multiply immediately, but need to be adjusted and adapted for a period of time to synthesize a variety of enzymes and improve the enzyme system in the body and other components of the cell. A shorter delay period indicates a stronger ...

Embodiment 3

[0076] Example 3 Using Hoagland nutrient solution as N eutrophication liquid to implement plant-microbe joint restoration

[0077] Collection method of umbrella grass root exudates: Weigh about 10g of umbrella grass, rinse the root system of umbrella grass several times with deionized water, then use filter paper to dry the surface water, put the plant into 200mL distilled water, at a temperature of ( 25±l) ℃, light intensity of 4000Lx, relative humidity of 75-80% in the artificial climate chamber for about 6 hours, after taking out the plants, filter the root exudate solution immediately, use a rotary evaporator to concentrate to 20ml, -20 Store at ℃ for later use, or directly save for later use.

[0078] Choose the same growth, in the Hoagland nutrient solution (Ca(NO 3 ) 2 ,945mg / l;KNO 3 ,607mg / l; (NH 4 ) 3 PO 4 ,115mg / l; MgSO 4 ,493mg / l; 2.5ml / l iron solution: FeSO4 ·7H 2 O,5.56g / l;Na 2 EDTA, 7.46g / l; 5ml / l Trace elements: sodium borate, 6.2mg / l; MnSO 4 ·H 2 O,2...

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Abstract

The invention discloses denitrifying bacteria and an aquatic plant-microbe combined repair method using the same. The denitrifying bacteria LR were collected in China Center for Type Culture Collection on May 3rd, 2011, and the collection number of the strains is CCTCC NO: M2011159. The plant-microbe combined repair method for a nitrogen eutrophic water body comprises the following steps of: planting cyperus alternifolius in the nitrogen eutrophic water body, and inoculating the denitrifying bacteria LR. The denitrifying bacteria LR and the cyperus alternifolius or cyperus alternifolius root exudates are combined for repairing the nitrogen eutrophic water body, and the strains LR has a promotion effect of repairing the nitrogen polluted water body through the cyperus alternifolius. The method for repairing the nitrogen eutrophic water body by combining the denitrifying bacteria LR and the cyperus alternifolius or the cyperus alternifolius root exudates is particularly suitable for treating the nitrogen eutrophic water body in nature.

Description

technical field [0001] The invention belongs to the technical field of environmental (water body) pollution control, and relates to a denitrifying bacterium and a combined water plant-microorganism restoration method thereof, in particular to a denitrifying bacterium and a plant-microbe used for water body nitrogen eutrophication Microbial joint remediation method. Background technique [0002] With the development of social economy, river pollution is a problem faced by all countries in the world. Treatment of urban polluted water bodies is a basic measure to protect urban water resources and improve the ecological environment. However, the commonly used physical and chemical remediation methods are generally expensive in investment, complicated in equipment and not suitable for remediation and treatment of large-scale water pollution. However, microbial (denitrifying bacteria) remediation technology cannot be widely used due to the limitation of bacterial colony growth b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/32C12R1/38
CPCY02W10/10
Inventor 陈亚华李星惠冯志航毛颖陆辰晨夏妍王桂萍沈振国
Owner NANJING AGRICULTURAL UNIVERSITY
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