Molecular marker miR-147a of breast cancer
A molecular marker and breast cancer technology, applied in the field of tumor diagnosis, can solve the problems of miRNA marker accuracy, insufficient sensitivity, inability to provide qualitative diagnosis, inability to display the whole picture of lesions, etc., to achieve easy mass screening and high specificity , The effect of convenient material collection
Active Publication Date: 2013-04-03
天津旷博同生生物技术有限公司
View PDF4 Cites 0 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Mammography is simple, convenient, non-invasive, and low-cost. It is one of the preferred methods for breast cancer screening. It displays the size, number, location, density, edge, shape, presence of calcification, and calcification of the tumor. The shape, size, number, steps, and surrounding halos and skin changes provide localization and qualitative signs and determine the nature of the lesion; its limitations are: (1) When the patient's breast glands are abundant, they overlap with the lesion and cannot Show the whole picture of the lesion, even false negative, (2) It is easy to miss the diagnosis of small tumors close to the chest wall and dense breast, (3) Sometimes it cannot provide a clear qualitative diagnosis
Although some studies have been carried out in this field, the accuracy and sensitivity of the existing miRNA markers are insufficient, and there is still a need to find more accurate and sensitive breast cancer miRNA markers in clinical and research
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment Construction
[0017] 1. Extraction of total RNA from serum
[0018] 2ml of blood was drawn from 5 breast cancer patients before operation and 7 days after operation. At the same time, 2ml of blood from 5 normal people was drawn as a normal control. After coagulation, centrifugation was performed. Finally, 1ml of the upper serum was taken and placed in 1.5ml of RNase / DNase-free. in a centrifuge tube.
[0019] Total RNA was extracted from serum using an RNA extraction kit (Beijing Kuangbo Biotechnology Co., Ltd.), and 1 μl (20 nM) of External Control- 1 (synthesized by Shanghai Sangon Bioengineering Technology Co., Ltd.) to monitor the quality of RNA extraction in serum. The concentration of extracted total RNA was determined using Thermo NanoDrop 2000c.
[0020] 2. Three-step quantitative detection of miRNA in serum
[0021] (1) Add polyA tail:
[0022] i. Prepare the reaction solution with polyA tail in an RNase-free PCR tube (Axygen, 200 μl), and the system is 20 μl. Add 1 μl (20 nM) ...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention provides a molecular marker miR-147a of breast cancer and use thereof in a diagnostic reagent for diagnosing breast caner. Content of miR-147a in serum of breast cancer patient is raised compared with that of a normal person. After the patient receives operation, the content of miR-147a in serum falls back to normal level. The invention further provides a diagnostic reagent kit for diagnosing breast caner. By utilizing the molecular marker miR-147a of breast cancer to diagnose breast caner, the characteristics of simplicity in operation, conveniently-obtained materials, safety, no invasiveness, high specific, high sensitivity and easiness for screening in a large scale are achieved.
Description
technical field [0001] The invention relates to the field of tumor diagnosis, in particular to a breast cancer molecular marker miR-147a, which can be applied to the screening of breast cancer high-risk groups, the identification of breast cancer, the monitoring of breast cancer treatment status and the monitoring of breast cancer prognosis and other fields. Background technique [0002] MicroRNA (miRNA) is a research hotspot in recent years. It is a kind of single-stranded small RNA that widely exists in eukaryotes. It has no coding function, but it can bind to the flanking region of gene sequence to repress or inhibit the translation of target mRNA. , with a high degree of conservation, timing and tissue specificity. In 2008, Mitchell et al. first found that many miRNAs exist stably in plasma, and the level of miR-141 can be used as a diagnostic marker for prostate cancer. Subsequent studies also found a colon cancer diagnostic marker miR-92a and a liver injury monitorin...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/113C12Q1/68
Inventor 李启靖李同恩栗世铀
Owner 天津旷博同生生物技术有限公司