Method for improving resistance to jujube witches' broom disease of jujube tree through mediation of agrobacterium rhizogenes

A technology of Agrobacterium rhizogenes and jujube madness, applied in the field of improving the resistance of jujube trees to jujube madness, can solve the problems of manual castration, embryo abortion, breeding difficulties, etc., and achieve strong controllability

Inactive Publication Date: 2012-09-19
HEBEI AGRICULTURAL UNIV.
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Problems solved by technology

However, jujube flowers are small, artificial detasseling is difficult, fruit setting rate is low, and embryo abortion is serious, so it is difficult to carry out traditional hybrid breeding

Method used

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  • Method for improving resistance to jujube witches' broom disease of jujube tree through mediation of agrobacterium rhizogenes
  • Method for improving resistance to jujube witches' broom disease of jujube tree through mediation of agrobacterium rhizogenes
  • Method for improving resistance to jujube witches' broom disease of jujube tree through mediation of agrobacterium rhizogenes

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Embodiment Construction

[0023] Taking Dongzao mad tissue cultured seedlings as test materials, cut the tender stems into about 1 cm long stems, and use OD 600 Agrobacterium rhizogenes 30148 with a concentration of 0.6-1.0 was infected for 5-15 minutes, and then transferred to the co-culture medium MS+5.5g / L agar+30g / L sucrose+200μM acetosyringone (AS) for co-culture for 3-7d, and then transferred to Into medium MS+5.5g / L agar+30g / L sucrose+100mg / L ceftriaxone sodium (CRO), cultured for 60d at 25±2℃, light / darkness 14 / 10h, and screened jujube Transformed seedlings with no symptoms of madness. Transfer healthy seedlings to MS+5.5g / L agar+30g / L sucrose+0.2mg / L BA+0.1mg / L IBA (pH value 5.8) supplemented with CRO 50-100mg / L to continue culturing. Change the medium every 20 days, cultivate 10 generations to completely degerm the transformed seedlings, then extract the genomic DNA of the transformed seedlings for rolC , rolD , tms PCR detection of genes and detection of jujube mad disease pathogen. ...

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Abstract

The invention relates to a method for improving resistance to jujube witches' broom disease of a jujube tree through mediation of agrobacterium rhizogenes, and the method comprises the following steps of: taking a stem section of tissue culture seedlings of the jujube tree infected by jujube witches' broom disease, infecting with agrobacterium rhizogenes, screening and transferring healthy tissue culture seedlings in the regenerated tissue culture seedlings; then continuously culturing and removing bacteria, detecting molecules of the transferred healthy tissue culture seedlings to determine transformed seedlings without causative agent of the jujube witches' broom disease; detecting and verifying the disease resistance of the transformed seedlings by micro-grafting technology; and finally obtaining the resistant seedlings of the jujube tree with remarkably improved resistance to jujube witches' broom disease. The method is simple and practicable and the resistant seedlings of the jujube tree with remarkably improved resistance to jujube witches' broom disease can be obtained within months.

Description

technical field [0001] The invention relates to a method for improving the disease resistance of jujube trees to jujube madness through the mediation of Agrobacterium rhizogenes. Background technique [0002] Jujube witches' broom disease (JWB) is a devastating quarantine disease caused by Phytoplasma. Jujube madness manifests as arbuscules, yellowing, and distortion of flowers, leaves, and roots on the entire plant. Symptoms of diseased branches are clumps, slenderness, and shortened internodes. The arbuscular branches are caused by abnormal germination of diseased buds and reversion of flower organs. When jujube madness occurs, symptoms usually appear on local branches from the flowering stage, and then gradually spread to the whole plant. Jujube trees lose their ability to bear fruit soon after the onset of the disease, and most of them die in about 3 years. Every year, more than ten million trees die due to jujube madness in the whole country, and the annual direct ec...

Claims

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Application Information

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IPC IPC(8): C12N15/82A01H5/00
Inventor 刘孟军赵锦郝征
Owner HEBEI AGRICULTURAL UNIV.
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