Preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid

A chondroitin sulfate and cartilage sulfate technology, which is applied in the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., can solve the problems of few reports on the separation and purification of CS oligosaccharides, and few studies on single oligosaccharides.

Inactive Publication Date: 2012-09-19
JIANGNAN UNIV
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  • Summary
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Problems solved by technology

At present, domestic research on CS mainly focuses on the extraction, physiological activity and derivatives of CS. There is no report on

Method used

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  • Preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid
  • Preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid
  • Preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid

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Embodiment 1

[0032] 1. Preparation of chondroitin sulfate oligosaccharides by enzymatic degradation

[0033] Dissolve 0.2 g of chondroitin sulfate in 10 mL of phosphate buffer with pH 5.9, and place it at 37 °C for 10 min to make it consistent with the reaction temperature, then add hyaluronidase (HAase, from bovine testis), making its enzyme activity 1.4×10 5 u / L, 150 rev / min shaking reaction for 22h. After the reaction, heat and boil for 20 minutes to inactivate the enzyme, centrifuge at 4000r / min for 15 minutes, take the supernatant and add 3 times the volume of 95% ethanol to precipitate, let it stand, dehydrate with 10mL absolute ethanol for 3 times, and vacuum dry to obtain chondroitin sulfate low Glycan A, and the total antioxidant activity (TOA) of the resulting degradation products was determined.

[0034] 2. Determination of total antioxidant activity

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Abstract

The invention discloses a preparation method for disaccharide, tetrasccharide and hexaose of chondroitin sulfuric acid, and belongs to the technical field of food. The method comprises the following steps that: the chondroitin sulfuric acid adopted as a raw material is degraded by hyaluronidase, so as to obtain chondroitin sulfuric acid oligosaccharide; through filtering separation by G-25 gel, desalination by G-10, DEAE cellulose 52 anion exchange chromatographic column separation, natural preparation of gel electrophoretic separation, and the like, the disaccharide, tetrasccharide and hexaose of the chondroitin sulfuric acid can be prepared. The invention relates to the preparation method for the disaccharide, the tetrasccharide and the hexaose of the chondroitin sulfuric acid. According to the invention, simultaneous preparation of three types of chondroitin sulfuric acid oligosaccharide can be firstly achieved, and a technical base for industrial preparation for single degree of polymerization chondroitin sulfuric acid oligosaccharide can be provided.

Description

technical field [0001] A method for preparing chondroitin sulfate disaccharide, tetrasaccharide and hexasaccharide belongs to the technical field of food industry. Background technique [0002] Chondroitin sulfate (CS), as a kind of glycosaminoglycan, is one of the three most important glycosaminoglycans. It is composed of D-glucuronic acid and N-acetyl-D-galactosaminic acid sulfate, and is a kind of acidic mucopolysaccharide widely present in cartilage tissues such as larynx, nasal septum and trachea of ​​humans and animals. CS with high molecular weight exhibits high biological activities, such as anti-cancer, anti-atherosclerosis, anti-inflammation, immune regulation, anti-oxidation, etc., not only in biological processes such as cell migration, differentiation, proliferation, recognition, and tissue formation. It has played an important role and has been used in medicine and clinical applications. However, due to high molecular weight, high apparent viscosity, low wate...

Claims

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Application Information

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IPC IPC(8): C12P19/14C07H1/06C07H11/00C08B37/08C25B7/00
Inventor 金征宇王金鹏张莲徐学明谢正军赵建伟周星焦爱权杨哪陈晓明田耀旗
Owner JIANGNAN UNIV
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