Primer special for detecting wheat spike branching suppression related gene and application of primer
A wheat and branching technology, applied in the field of molecular biology, can solve the problems of increasing workload, failing to obtain wheat branching gene molecular markers, delaying the breeding speed, etc. Effect
Inactive Publication Date: 2012-09-19
张卫东
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Problems solved by technology
[0020] (4) At present, in hexaploid common wheat, no branched genes and effective molecular markers of wheat have been obtained
[0021] (5) Problems in wheat branch breeding at present: Since the common cultivated wheat is hexaploid, the branching traits are controlled by two recessive genes. Lines, in the selection of offspring, self-selection is required to determine whether they have branched phenotypes, so the breeding speed is delayed and the workload is increased
Method used
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Embodiment 1
[0044] 1. Acquisition of the special primer TFL237 for detecting genes related to panicle branch suppression in wheat
[0045] Isolation and Transformation of Genes
[0046] The young spikes from the two-row stage to the floret differentiation stage of wheat branch varieties Zhizhimai and Taishan 008 were used as materials, and the differential fragments were obtained by analyzing the results of cDNA subtractive hybridization, one of which was a LEY / FLO-like gene fragment It may be related to branching; the specific steps are as follows:
[0047] (1) RNA extraction and mRNA isolation and purification:
[0048] Take the young ears from the two-row stage to the floret differentiation stage of the branched wheat variety branched wheat and the normal ear type Taishan 008 respectively, put them in a liquid nitrogen precooled mortar, add liquid nitrogen to quickly grind them into powder, and put the powder into Into a 1.5ml centrifuge tube, quickly add 1ml Trizol reagent, invert a...
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The invention discloses a primer special for detecting wheat spike branching suppression related gene and application of the primer. The special primer consists of a pair of oligonucleotides of which the nucleotide sequences are shown as SEQ ID NO.1 and SEQ ID NO.2. The invention also discloses application of the special primer to detection that whether wheat has spike branching suppression related gene. The primer special for detecting the wheat spike branching suppression related gene can be used for early generation selection of wheat breeding, the workload of breeding screening is greatlyreduced, a breeding period is shortened, a breeding speed is improved, breeding cost is reduced, and the primer has the advantages of simple operation, low cost and short period, is suitable for promotion and application, and provides technical support for wheat breeding, and a quick selection method for breeding high-yield wheat germplasm is provided.
Description
technical field [0001] The invention discloses a special primer for detecting genes related to wheat panicle branch suppression and an application thereof, belonging to the technical field of molecular biology. Background technique [0002] In the final analysis, plant breeding is the improvement of the chromosomal and extrachromosomal genetic structure of species (variety). At this stage, the use of conventional breeding methods for breeding work is still the main way. The main methods include selective breeding and cross breeding. But we will encounter some insurmountable difficulties in the conventional breeding process. For example, it is inconvenient to identify genotypes when the alleles are not obvious in appearance, the alleles are recessive alleles, and there is an interaction between the allele and other genes or the environment; the equivalent phenotype Identification is also quite troublesome; early selection cannot be carried out; wider and stronger selection...
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IPC IPC(8): C12Q1/68C12N15/11
Inventor 张卫东田纪春李达赵兰飞宋玉刚
Owner 张卫东