Beta-cyclodextrin clathrating technique for borneol in Saimeian emulsifiable paste
A cyclodextrin and borneol technology, which is applied in the field of β-cyclodextrin package and technology, can solve the problems of borneol content decline, unstable quality, borneol volatilization, etc.
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Embodiment 1
[0029] Dissolve borneol in an appropriate amount of ethanol, and dissolve β-cyclodextrin with 4 times the mass of borneol in purified water at 40°C to make a saturated solution; slowly pour the dissolved borneol into the saturated solution of β-cyclodextrin, at 40°C , stirred at a stirring speed of 600r / min for 2h, then placed at 4°C for 24 hours, then filtered with suction, and washed the clathrate with a small amount of ethanol to remove unincluded borneol; filtered again, and the obtained The clathrate was placed in a vacuum drying oven at 25 degrees Celsius and dried for 24 hours to obtain the β-cyclodextrin borneol clathrate.
Embodiment 2
[0031] Dissolve borneol in an appropriate amount of ethanol, and dissolve β-cyclodextrin with 6 times the mass of borneol in purified water at 43°C to make a saturated solution; slowly pour the dissolved borneol into the saturated solution of β-cyclodextrin, at 43°C , stirred at a stirring speed of 700r / min for 3h, then placed at 4°C for 24 hours, then filtered with suction, and washed the clathrate with a small amount of ethanol to remove unclathed borneol; filtered again, and the obtained The clathrate was placed in a vacuum drying oven at 25 degrees Celsius and dried for 24 hours to obtain the β-cyclodextrin borneol clathrate.
Embodiment 3
[0033] Dissolve borneol in an appropriate amount of ethanol, and dissolve β-cyclodextrin with 9 times the mass of borneol in purified water at 45°C to make a saturated solution; slowly pour the dissolved borneol into the saturated solution of β-cyclodextrin, at 45°C , stirred at a stirring speed of 900r / min for 4.5h, then placed at 4°C for 24 hours, then filtered with suction, and washed the clathrate with a small amount of ethanol to remove unincluded borneol; filtered again to obtain The clathrate was placed in a vacuum oven at 25 degrees Celsius and dried for 24 hours to obtain the clathrate of β-cyclodextrin borneol.
[0034] One of the verification schemes of the present invention:
[0035] Thin-layer chromatography: Weigh 130mg of β-cyclodextrin borneol inclusion complex, put it in a 15ml stoppered centrifuge tube, add 10ml of absolute ethanol, shake and mix, ultrasonicate for 20min, centrifuge at 3000r / min for 10min, and take the supernatant; Then take 100mg of borneol...
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