Culture medium and preparation method thereof, microbial material and application and preparation method thereof

A medium and microorganism technology, applied in the biological field, can solve problems such as impossible application in practical applications, and achieve the effects of improving fruit taste, high quality and simple preparation method

Active Publication Date: 2012-09-26
株式会社缘织
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the medium (i.e., agar medium) constituted in this way, although useful in research laboratories, cannot be applied to practical applications in any case.

Method used

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  • Culture medium and preparation method thereof, microbial material and application and preparation method thereof
  • Culture medium and preparation method thereof, microbial material and application and preparation method thereof
  • Culture medium and preparation method thereof, microbial material and application and preparation method thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0049] The preparation process of the mineral mother liquor is described in detail below. The above-mentioned mineral mother liquors are made based on special mother liquors and minerals.

[0050] The preparation process of described special mother liquor sees image 3 .

[0051] The first step is to prepare a brown sugar solution containing bromelain: place bromelain in an aqueous brown sugar solution with a concentration of 7-15% by mass to obtain a brown sugar solution with a bromelain concentration of 60-100 U / mL;

[0052] The second step, the mixing process of microorganisms: in this process, the microorganisms of the genus Flavobacterium (Flavobacterium), microorganisms of the genus Pseudomonas, and Aspergillus are inoculated into the black sugar solution containing bromelain obtained in the first step. Microbes of the genus Aspergillus, microorganisms of the genus Rhizopus and bulgaric acid bacteria, wherein the weight ratio of the microbial liquid of the genus Flavob...

Embodiment 1

[0062] First, grind into 250-350 mesh chlorite powder (60 parts by weight), pegmatite powder (20 parts by weight), Duanhu silica powder (10 parts by weight), amphibole powder (9 parts by weight) and rare earth Powder (1 part by weight) was mixed. Then spray the above-mentioned mineral mother liquor (40 parts by weight) therein, heat and stir while spraying the above-mentioned mineral mother liquor, and mix uniformly. Then put it into a sealed container, and cultivate it at about 30° C. for 72 hours to obtain the culture medium of the present invention.

Embodiment 2

[0064] Grinding into 250~350 mesh chlorite powder (50 parts by weight), pegmatite powder (25 parts by weight), Duanhu silica powder (15 parts by weight), phyllite powder (8 parts by weight) and rare earth powder ( 2 parts by weight) mixed. Then spray the mineral mother liquor (35 parts by weight) therein, heat and stir while spraying the mineral mother liquor, and mix evenly. Then put it into a sealed container, and cultivate it at about 30° C. for 85 hours to obtain the culture medium of the present invention.

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Abstract

The invention provides a culture medium and belongs to the field of biotechnology. The culture medium is applied to the field and is prepared from porous stone powder, rare earth powder and a mineral mother solution, wherein the mineral mother solution is 35 to 45 percent of the total weight of the porous stone powder and the rare earth powder. The invention discloses a microbial material generated by the culture medium, and application of the microbial material. The microbial material can convert organic waste into a biological organic fertilizer. The microbial material is easy and convenient to use, low in cost and full in function, and can be used on a large scale; in addition, when the microbial material is used for fermenting the organic waste, the fermentation time is short, the method is simple, and compared with composting, the method has the advantages that the fermentation time can be shortened by 5-10 days and a step of crushing the organic waste can be eliminated; and the biological organic fertilizer prepared from the microbial material has high quality, can improve yield per mu, and improves the mouthfeel of fruits, and a continuous cropping effect is not reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a culture medium and a preparation method thereof, a microbial material and its use and preparation method, and the microbial material can convert organic waste into bio-organic fertilizers. Background technique [0002] The opposite of today's rich consumer life can cause profound waste disposal problems. Although combustible waste is relatively easy to dispose of, it is not so easy to dispose of organic waste such as food waste. In addition to household food waste, organic waste includes animal manure, remains of marine organisms, silt, fish and animal offal from food processing factories, etc. In addition, there are more and more various nutritious drinks, etc., and their raw materials are mainly organic matter such as vegetables, fruits, seaweed, etc., but their residues have not been effectively used, nor can they be simply processed, so they become We usually say "very tricky...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/20C05F9/04C12R1/20C12R1/38C12R1/66C12R1/845C12R1/225
CPCY02A40/20Y02W30/40
Inventor 大菅秀信市川真臣杜京忠白石俊行
Owner 株式会社缘织
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