Method for preparing biological organic fertilizer by utilizing wastewater separate substances of livestock and poultry
A bio-organic fertilizer and separation technology, which is applied in the field of agricultural waste resource recycling, can solve problems such as weakness, and achieve the effects of promoting absorption, low cost, and simple and easy production process.
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example 1
[0038] Preparation of secondary isolates:
[0039] 1) Livestock and poultry waste water is poured into the regulating tank, and then 0.01-3% of additives are added according to the weight percentage for aeration and stirring. At the same time, ultrasonic treatment is used. The ultrasonic frequency range is 20-100kHZ, and the ultrasonic intensity range is 0.1-70W / cm 2 , Ultrasonic response time 0.1 ~ 60min. The auxiliary agent is one or a mixture of deodorants and microbial regulators.
[0040] Ultrasonic treatment preferably has an ultrasonic frequency of 40kHZ and an ultrasonic intensity of 40W / cm 2 , Ultrasonic response time is 3min.
[0041] (2), the waste water of the regulating tank after the treatment of step (1) is separated through a grid coarse sieve, and large particles are removed to obtain a primary isolate. The waste water of the regulating pond is injected into the separation tank, and 0.01~ 3% of the auxiliary agent is aerated and stirred for aerobic treatmen...
Embodiment 2
[0064] Preparation of secondary isolate step is the same as in Example 1
[0065] The preparation of bio-organic fertilizer consists of the following components:
[0066] 25kg of secondary isolate, 69kg of mushroom dregs, 3kg of bacteria agent for the first fermentation, and 3kg of bacteria agent for the second fermentation.
[0067] Make the first fermentation bacteria agent:
[0068] Inoculate Bacillus cereus in LB medium and culture in a test tube at a culture temperature of 25-35°C and a culture time of 36-60 hours;
[0069] The Bacillus cereus cultured in the test tube is transferred to a 1000ml shake flask for culture, the culture temperature is 22-35°C, the rotation speed is 160-200 rpm, and the culture time is 24-48 hours;
[0070] Then, the Bacillus cereus cultivated in the shake flask is inserted into the seed tank for cultivation, the cultivation temperature is 25-35° C., and the cultivation time is 24-48 hours;
[0071] After being cultivated in the seed tank, t...
Embodiment 3
[0084] Preparation of secondary isolate step is the same as in Example 1
[0085] The preparation of bio-organic fertilizer consists of the following components:
[0086] 40kg of secondary isolate, 56kg of straw powder, 2kg of bacteria agent for the first fermentation, and 2kg of bacteria agent for the second fermentation.
[0087] Make the first fermentation bacteria agent:
[0088] Inoculate Bacillus cereus in LB medium and culture in a test tube at a culture temperature of 25-35°C and a culture time of 36-60 hours;
[0089] The Bacillus cereus cultured in the test tube is transferred to a 1000ml shake flask for culture, the culture temperature is 22-35°C, the rotation speed is 160-200 rpm, and the culture time is 24-48 hours;
[0090] Then, the Bacillus cereus cultivated in the shake flask is inserted into the seed tank for cultivation, the cultivation temperature is 25-35° C., and the cultivation time is 24-48 hours;
[0091] After being cultivated in the seed tank, the...
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