Compounds for detecting content of micro magnesium ions and application thereof

Abstract

The invention relates to compounds for detecting the content of micro magnesium ions as well as a preparation method and an application. The compounds for detecting the content of the micro magnesium ions adopt the following structural general formula, wherein R1, R2 and R3 are arbitrary groups; and Y is functional groups with fluorescence developing. The compounds for detecting the content of the micro magnesium ions leads chromophore in 2-hydroxypyridine-3-formic acid, so that the fluorescence efficiency of molecules is improved, fluorescence response is more obvious when the detection range is narrowed down, and the detection sensitivity is improved at the same time. When the content of the magnesium ions changes, the fluorescence intensity of the magnesium ions is changed along with the content, so that the content of the magnesium ions in solution can be calculated according to the fluorescence intensity of the magnesium ions, the process analysis of the content of the micro magnesium ions can be achieved, and the efficiency, the sensitivity and the repeatability can be improved greatly; and in addition, the compounds can be used for detecting the content of the magnesium ions in new technical researches in various fields, the application range of the compounds can be enlarged greatly, and the compounds are convenient for people to use.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a compound for content detection, in particular to a compound for trace magnesium ion content detection and application thereof. Background technique [0002] Magnesium ions are the most abundant divalent cations in organisms, and their concentration changes are closely related to the activities of many enzymes in organisms, especially in the synthesis of DNA and phosphorylation of proteins. In addition, the detection of magnesium ion concentration is also of great significance to the environment, especially to the marine environment. For the content of trace magnesium ions, it is difficult to accurately determine its content by using traditional detection methods such as EDTA complexometric titration, and it is also difficult to realize the detection of the millimolar concentration range. [0003] At present, the fluorescent probes for magnesium ions in vivo mainly use o-Amino...

AI Technical Summary

Problems solved by technology

There are very few reports on the detection of extracellular magnesium ion concentration. Recently, there have been reports on the detection method of fluorescent probes for magnesium ions, such as a β-diketone ion complex based on a β-diketone ion complex reported by Hirokazu Komatsu, which can be used for extracellular ion detection. fluorescent probe, but its detection limit is high, it is not sensitive to low concentration of magnesium, and the molecule does not contain functional groups that can be used for immobilization, so it cannot be used to make reversible sensors, so it cannot be applied to various applications that require multiple uses. Continuous detection of magnesium ions in various samples, blood or seawater

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