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New hyaluronic acid strain rejuvenation method

A technology of hyaluronic acid and a new method, applied in the directions of biochemical equipment and methods, microorganisms, microorganisms, etc., can solve the problems of insufficient bacterial species, low rejuvenation success rate, easy degradation, etc. The effect of increased acid rate and good performance

Inactive Publication Date: 2013-08-28
柳州盛强生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The hyaluronic acid strains preserved in actual production or in the laboratory often degenerate. Therefore, continuous rejuvenation is required to obtain strains with excellent performance. The conventional rejuvenation method is to use conventional or enriched medium plates to carry out The streak separation method uses the elimination method to separate the strains, and selects the strains with good performance to rejuvenate them. However, this method is conservative, and the success rate of rejuvenation is very low, and the strains obtained from rejuvenation are not good enough and easy to degrade

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A new method for rejuvenating hyaluronic acid strains, comprising the following steps:

[0023] (1) Preparation of rejuvenating agent:

[0024] a1. First take 150 grams of fresh potatoes and chop them into granules with a particle size of less than 5mm, then put the potatoes and 50 grams of fresh potatoes into a soymilk machine and grind them, then add them to a 1800mL beaker with 400ml of water and mix evenly;

[0025] b1. Heat the above mixture until the water boils, keep the material rolling up and down, cover the beaker with a glass sheet to prevent excessive evaporation of water, keep the boiling time for about 3 hours, and stir the material every 20 minutes to prevent evaporation Coke, evaporate 50%-70% (volume ratio) of water to obtain 300-400mL mixed solution;

[0026] c1. Filter out 120mL of the extract with a sterilized filter cloth in an aseptic operation on the aseptic operating table, and cool to normal temperature for later use;

[0027] d1. Add 30mL of ...

Embodiment 2

[0034] A new method for rejuvenating hyaluronic acid strains, comprising the following steps:

[0035] (1) Preparation of rejuvenating agent:

[0036] a1. Take 225 grams of fresh potatoes and chop them into small pieces (less than 5mm), then put the potatoes and 75 grams of fresh potatoes into a soybean milk machine and grind them, then add them to a 1800mL beaker with 600ml of water and mix well ;

[0037] b1. Heat the above mixture until the water boils, keep the material rolling up and down, cover the beaker with a glass sheet to prevent excessive evaporation of water, keep boiling for 2.5-3.5 hours, and stir the material every 20 minutes to prevent Coke, evaporate 50%-70% (volume ratio) of water to obtain 450-600mL mixed solution;

[0038] c1. Filter out 160mL of the extract with a sterilized filter cloth in an aseptic operation on the aseptic operating table, and cool to normal temperature for later use;

[0039] d1. Add 40mL of sterile defibrated sheep blood to the ab...

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PUM

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Abstract

The invention relates to a new hyaluronic acid strain rejuvenation method. The method prepares a rejuvenation agent from a common animal material (sheep blood) and a plant material (potato and corn) through a special treatment method, and comprises the following steps of: mixing and grinding the potato and corn, and adding water; heating to boiling for 2.5-3.5 hours, and evaporating the water accounting for 50-70% of the mixture volume; filtering to obtain the extract liquid for later use; finally adding the sterile defiber sheep blood into the extract liquid, and uniformly mixing to obtain the rejuvenation agent; adding the rejuvenation agent into a hyaluronic acid strain selection solid medium according to a certain proportion to obtain a rejuvenation flat; inoculating the degraded strain into the flat by use of an inoculation ring, and streaking; and culturing to obtain the hyaluronic acid strain with good performance. According to the invention, the raw materials of the rejuvenation agent are easily available, and the preparing and using process is simple; and through the method, the rejuvenation probability of the strain is improved by over 10 times, the obtained strain has good performance, and the acid yield is improved by about 30%.

Description

technical field [0001] The invention relates to a method for rejuvenating strains, in particular to a new method for rejuvenating hyaluronic acid strains. Background technique [0002] The hyaluronic acid strains preserved in actual production or in the laboratory often degenerate. Therefore, continuous rejuvenation is required to obtain strains with excellent performance. The conventional rejuvenation method is to use conventional or enriched medium plates to carry out The streak separation method uses the elimination method to separate the strains, and selects the strains with good performance to rejuvenate them. However, this method is conservative, and the success rate of rejuvenation is very low, and the strains obtained from rejuvenation are not good enough and easy to degrade . Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a new method for the rejuvenation of hyaluronic acid strains with easy-to-obtain ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00
Inventor 欧韦福刘树新黄云兵
Owner 柳州盛强生物科技有限公司