Detergent compositions containing thermobifida fusca lipase and methods of use thereof

A composition and detergent technology, applied in the direction of detergent composition, detergent compounding agent, chemical instrument and method, etc., can solve the problem of synthesizing complex toxic waste products, etc.

Inactive Publication Date: 2012-10-03
DANISCO US INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Preferably, these enzymes will utilize naturally occurring or commonly used starting materials and will not require protection and deprotection steps in the synthetic reaction that would complicate the synthesis and lead to the generation of toxic waste products

Method used

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  • Detergent compositions containing thermobifida fusca lipase and methods of use thereof
  • Detergent compositions containing thermobifida fusca lipase and methods of use thereof
  • Detergent compositions containing thermobifida fusca lipase and methods of use thereof

Examples

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example

[0184] The following examples are provided to demonstrate and illustrate certain preferred embodiments and aspects of the invention and should not be construed as limiting.

[0185]In the following experimental disclosures, the following abbreviations will be used: M (molar); mM (millimolar); μM (micromolar); nM (nanomolar); μmol (micromole); nmol (nanomole); gm (gram); mg (milligram); μg (microgram); pg (picogram); L (liter); ml and mL (milliliter); μl and μL (microgram); liter); cm (centimeter); mm (millimeter); μm (micrometer); nm (nanometer); U (unit); MW (molecular weight); sec (second); min(s) (minute); h(s) and hr(s) (hours); °C (degrees Celsius); QS (sufficient); ND (not performed); rpm (revolutions per minute); H 2 O (water); dH 2 O (deionized water); HCl (hydrochloric acid); aa (amino acid); bp (base pair); kb (kilobase pair); kD (kilodalton); MgCl 2 (magnesium chloride); NaCl (sodium chloride); w / v (weight / volume ratio); v / v (volume ratio); g (gravity); OD (optic...

example 1

[0187] Cloning and Expression of Lipase 2 (TfuLip2) from Thermobista spp.

[0188] The Thermobista spp. lipase 2 (or BTA-hydrolase 2) gene has been previously identified (Lykidis et al., J. Bacteriol, 189:2477-2486, 2007) , whose sequence is shown in GENBANK accession number YP_288944. The Bacillus subtilis expression vector p2JM103BBI (Vogtentanz et al., Prot. Expr. Purif., 55:40-52, 2007) was digested with restriction enzymes BssHII and HindIII. A DNA fragment lacking the sequence of the BCE103-BBI fusion gene was isolated and used as an expression backbone. This fragment was ligated to a synthetic gene encoding the TfuLip2 enzyme, resulting in a fusion of the N-terminus of the TfuLip2 polypeptide to the third amino acid of the B. subtilis AprE propeptide encoded by p2JM103BBI. The recombinant TfuLip2 protein produced in this way has three additional amino acids (Ala-Gly-Lys) at its amino terminus after cleavage by the native signal peptidase in the host.

[0189] The n...

example 2

[0197] Isolation and characterization of TfuLip2

[0198] Ultrafiltration concentrates were obtained from batch fermentation of B. subtilis expression strains on a 14 L scale. The clarified broth was used to characterize the recombinant TfuLip2 polypeptide.

[0199] To purify TfuLip2, the ultrafiltration concentrate was obtained from batch fermentation on a 14 L scale and diluted 5-fold with 50 mM Tris-HCl (pH 8.0) buffer, ammonium sulfate was added to a final concentration of 1 M. The precipitated pellet from ammonium sulfate precipitation was collected and used for further purification. A FastFlow Phenyl Sepharose column equilibrated with 1 M ammonium sulfate in 50 mM Tris-HCl (pH 8.0) buffer was used. Samples were loaded at half the equilibrium flow rate (12ml / min) and washed with equilibration buffer after loading. A gradient was used to decrease the concentration of ammonium sulfate in the buffer from 1M to 0M. Contaminating proteins in the column were washed with 5...

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Abstract

The present compositions and methods relate to a lipase cloned from Thermobifida fusca, polynucleotides encoding the lipase, and methods of use thereof. The compositions and methods have particular application in detergent cleaning compositions and methods.

Description

[0001] priority [0002] This application for patent claims the benefit of U.S. Provisional Application Serial No. 61 / 288,666, filed December 21, 2009, and U.S. Provisional Application Serial No. 61 / 350,747, filed June 2, 2010, whereby both Both applications are incorporated herein by reference in their entirety. technical field [0003] The compositions and methods of the present invention relate to lipases cloned from Thermobifida fusca, polynucleotides encoding the lipases, and methods of use thereof. Background technique [0004] Current laundry detergent and / or fabric care compositions include ingredients such as surfactants, enzymes (proteases, amylases, lipases and / or cellulases), bleaches, builder systems, suds suppressors Complex combinations of active ingredients such as , stain suspending agents, soil release agents, optical brighteners, softeners, dispersants, dye transfer inhibiting compounds, abrasives, bactericides and fragrances. [0005] Lipolytic enzymes,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11D3/386C12N9/20C12N9/18
CPCC12N9/20C12N9/18C11D3/38627
Inventor C·亚当斯B·施密特
Owner DANISCO US INC
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