Mesenchymal stem cell and application thereof in resisting HIV-1 (human immunodeficiency virus)
A kind of stem cell and cell technology, applied in the field of stem cell therapy, can solve the problem of irreparable thymus function, etc., and achieve the effect of enhancing immune function, uniform environment, easy measurement and monitoring
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0040] Example 1 High-density culture of umbilical cord mesenchymal stem cells
[0041] 1. The seed cells for high-density culture of umbilical cord mesenchymal stem cells come from umbilical cord mesenchymal stem cells produced by Tianjin Heze Stem Cell Technology Co., Ltd., batch number: G2012098, passage: P3 generation.
[0042] 2. Materials needed for high-density cell culture
[0043] (1) D-MEM / F-12 medium
[0044] (2) D-Hank's buffer
[0045] (3) Fetal bovine serum
[0046] (4) Microcarriers: Cytodex3 microcarriers produced by GE Healthcare were selected. The factory standard is: the microcarrier matrix is cross-linked dextran, and the surface of the carrier is covered with a thin layer of denatured collagen; the density is 1.04g / ml; the particle size is d 50 =175μm, d 5-95 =141μm-211μm; the culture area provided is 2700cm 2 / g dry weight; the number of microcarriers per gram dry weight is about 3.0×10 6 , The expansion factor is 15ml / g dry weight.
[0047] (...
Embodiment 2
[0086] Example 2 Umbilical cord mesenchymal stem cells promote thymus development in nude mice
[0087] 1. Experimental protocol
[0088]200 normal 3-week-old nude mice were randomly divided into a treatment group and an untreated group, 100 in each group, fed at SPF level, and the treatment group was intraperitoneally injected with 0.5ml of the umbilical cord mesenchymal stem cell (UC-MSCs) suspension obtained in Example 1 , 2×10 6 Cells / mouse, and the untreated group was intraperitoneally injected with 0.5ml D-Hank's solution, both twice a week. After injection, 6 nude mice / group were killed every two weeks, thymus tissue was taken, weighed, the starting time of thymus enlargement of nude mice was tracked, and the changes of body weight and thymus weight of nude mice were recorded over time. The thymus specimens were taken, and the thymus of 3 nude mice were frozen sections, and the change of the network structure composed of thymic epithelial cells in the thymus and the...
Embodiment 3
[0098] Example 3 Therapeutic Effect of Umbilical Cord Mesenchymal Stem Cells on Rhesus Monkeys Infected with SIVmac239 Virus
[0099] 1. Experimental protocol
[0100] Sixteen healthy adult Chinese rhesus monkeys were selected as model animals, including 12 males and 4 females. Molecular cloning standard strain SIVmac239 was used as the infection strain, and each animal was inoculated with 100TCID 50 / ml Infection was inoculated by intravenous route. When the animals were infected with the virus for 10 days and 56 days, they were randomly divided into 4 groups according to the viral load, body weight and sex of the animals, with 3 male animals and 1 female animal in each group. They were: model group (CP), PMPA treatment group (TA), stem cell therapy group (TB), stem cell + PMPA combined therapy group (TC). The administration of the control substance and the test substance was carried out according to the grouping situation, wherein the model control group: intravenous in...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com