Mesenchymal stem cell and application thereof in resisting HIV-1 (human immunodeficiency virus)

A kind of stem cell and cell technology, applied in the field of stem cell therapy, can solve the problem of irreparable thymus function, etc., and achieve the effect of enhancing immune function, uniform environment, easy measurement and monitoring

Active Publication Date: 2013-05-29
UNION STEMCELL & GENE ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs used to treat AIDS only target viral infection, not repair damaged thymus function

Method used

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  • Mesenchymal stem cell and application thereof in resisting HIV-1 (human immunodeficiency virus)
  • Mesenchymal stem cell and application thereof in resisting HIV-1 (human immunodeficiency virus)
  • Mesenchymal stem cell and application thereof in resisting HIV-1 (human immunodeficiency virus)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 High-density culture of umbilical cord mesenchymal stem cells

[0041] 1. The seed cells for high-density culture of umbilical cord mesenchymal stem cells come from umbilical cord mesenchymal stem cells produced by Tianjin Heze Stem Cell Technology Co., Ltd., batch number: G2012098, passage: P3 generation.

[0042] 2. Materials needed for high-density cell culture

[0043] (1) D-MEM / F-12 medium

[0044] (2) D-Hank's buffer

[0045] (3) Fetal bovine serum

[0046] (4) Microcarriers: Cytodex3 microcarriers produced by GE Healthcare were selected. The factory standard is: the microcarrier matrix is ​​cross-linked dextran, and the surface of the carrier is covered with a thin layer of denatured collagen; the density is 1.04g / ml; the particle size is d 50 =175μm, d 5-95 =141μm-211μm; the culture area provided is 2700cm 2 / g dry weight; the number of microcarriers per gram dry weight is about 3.0×10 6 , The expansion factor is 15ml / g dry weight.

[0047] (...

Embodiment 2

[0086] Example 2 Umbilical cord mesenchymal stem cells promote thymus development in nude mice

[0087] 1. Experimental protocol

[0088]200 normal 3-week-old nude mice were randomly divided into a treatment group and an untreated group, 100 in each group, fed at SPF level, and the treatment group was intraperitoneally injected with 0.5ml of the umbilical cord mesenchymal stem cell (UC-MSCs) suspension obtained in Example 1 , 2×10 6 Cells / mouse, and the untreated group was intraperitoneally injected with 0.5ml D-Hank's solution, both twice a week. After injection, 6 nude mice / group were killed every two weeks, thymus tissue was taken, weighed, the starting time of thymus enlargement of nude mice was tracked, and the changes of body weight and thymus weight of nude mice were recorded over time. The thymus specimens were taken, and the thymus of 3 nude mice were frozen sections, and the change of the network structure composed of thymic epithelial cells in the thymus and the...

Embodiment 3

[0098] Example 3 Therapeutic Effect of Umbilical Cord Mesenchymal Stem Cells on Rhesus Monkeys Infected with SIVmac239 Virus

[0099] 1. Experimental protocol

[0100] Sixteen healthy adult Chinese rhesus monkeys were selected as model animals, including 12 males and 4 females. Molecular cloning standard strain SIVmac239 was used as the infection strain, and each animal was inoculated with 100TCID 50 / ml Infection was inoculated by intravenous route. When the animals were infected with the virus for 10 days and 56 days, they were randomly divided into 4 groups according to the viral load, body weight and sex of the animals, with 3 male animals and 1 female animal in each group. They were: model group (CP), PMPA treatment group (TA), stem cell therapy group (TB), stem cell + PMPA combined therapy group (TC). The administration of the control substance and the test substance was carried out according to the grouping situation, wherein the model control group: intravenous in...

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Abstract

The invention discloses a mesenchymal stem cell and the application thereof in resisting HIV-1 (human immunodeficiency virus), and particularly relates to a mesenchymal stem cell of a secreted-expression high-concentration keratinocyte growth factor (KGF), and the mesenchymal stem cell is produced through a micro-carrier culturing system. According to the invention, the mesenchymal stem cell can be used for promoting the development of the thymus gland, maintaining the functions of the thymus gland, and enhancing the immunologic function of specific T cells through increasing the output of T lymphocytes to peripheral blood, thereby achieving an effect of treating HIV-1 infection, and providing an effective new strategy for the clinical treatment of the acquired immunodeficiency syndrome.

Description

technical field [0001] The invention relates to the field of stem cell therapy, in particular to a mesenchymal stem cell immunomodulator, the main component of which is mesenchymal stem cells secreting and expressing keratinocyte growth factor, which can be used for the treatment of HIV-1 infection. Background technique [0002] Mesenchymal Stem Cells (Mesenchymal Stem Cells, MSCs) are mesenchymal tissue-derived stem cells with subtotipotent differentiation potential, which can not only regulate the function of the peripheral immune system, but also repair the damaged central immune system. plays a vital role in its maintenance. In autoimmune diseases, after MSCs are injected into the body, they can not only migrate to injured tissues, inhibit the release of pro-inflammatory factors, promote the survival of injured cells, induce peripheral immune tolerance, reduce abnormal immune responses, but also repair damaged cells. Thymus tissue, thereby reducing the production of aut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0775C12N5/02A61K35/44A61K38/18A61K38/38A61P37/02A61P5/00A61P31/18A61K31/727A61K35/28
Inventor 刘拥军朱德琳李福彬牟浩王立华
Owner UNION STEMCELL & GENE ENG
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