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Method for increasing hydrogen yield of Chlamydomonas reinhardtii by using slow-growing Bradyrhizobium japomcum

A technology of soybean rhizobia and rhinoceros is applied in the field of biological hydrogen production, which can solve problems such as inability to meet industrial production.

Inactive Publication Date: 2012-10-17
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method also inhibits the electron production generated by photolysis of water, and ultimately still cannot meet the needs of industrial production. [3-5]

Method used

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  • Method for increasing hydrogen yield of Chlamydomonas reinhardtii by using slow-growing Bradyrhizobium japomcum
  • Method for increasing hydrogen yield of Chlamydomonas reinhardtii by using slow-growing Bradyrhizobium japomcum
  • Method for increasing hydrogen yield of Chlamydomonas reinhardtii by using slow-growing Bradyrhizobium japomcum

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Experimental program
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Effect test

Embodiment 1

[0043] The growth situation of rhizobia co-cultured with cc849 and lba under the normal conditions of embodiment 1

[0044] In the first stage of the two-step hydrogen production technology, in order to study the influence of rhizobia on the growth of Chlamydomonas, rhizobia were mixed with Chlamydomonas reinhardtii cc849 and transgenic algae lba in normal TAP according to the volume ratio of 2ml:30ml. In the medium, the pure cc849 and lba were used as controls respectively, and the number of algae cells in the culture medium was detected every day (such as figure 1 shown), the results showed that when cc849 and lba were co-cultured with rhizobia, the time dynamic trend of their growth was similar to that of cc849 and lba when they were cultured alone: ​​the algal cells entered the logarithmic growth phase on the first day of culture, and in the It reaches saturation in about 3-4 days, and then tends to be stable. However, the number of algal cells co-cultured with rhizobia a...

Embodiment 2

[0045] The effect of rhizobia on the hydrogen production of cc849 and lba under the condition of hydrogen production in the absence of sulfur in Example 2

[0046] In the second stage of the two-step hydrogen production technology, rhizobia were mixed with cc849 and lba in sulfur-deficient medium according to different volume ratios for co-culture, and pure cc849 and lba were cultured separately as controls, and hydrogen production was continuously detected Quantitative differences (such as figure 2 shown). From figure 2 It can be seen that rhizobia significantly promoted the accumulation of hydrogen production of cc849 and lba, and the promoting effect was related to the volume ratio of algal bacteria. When the concentration of rhizobia is OD 600 When the cell concentration of =1, cc849 and lba was 12.5mg chlorophyll / L, rhizobia was in algae liquid: when bacterium liquid (volume ratio) was 40: 1, promptly added 1mL bacterium liquid in 40mL algae liquid, the production of...

Embodiment 3

[0047] Photosynthetic oxygen release and respiratory oxygen consumption of the co-culture system of embodiment 3 algae

[0048] In normal TAP medium, the effects of co-cultivation of rhizobia with cc849 and lba on photosynthetic oxygen evolution and respiration oxygen consumption in the system were detected by oxygen electrode method. The results showed that no photosynthetic oxygen evolution could be detected when rhizobia were co-cultured with Chlamydomonas cc849 and lba, only respiratory oxygen consumption (such as image 3 shown). In the first 3 days of 7-day continuous culture, the respiration rate of cc849 and lba cultured alone showed a gradual downward trend, and reached the lowest on the third day, about 4.22-4.30 μmol O 2 .mg -1 chl.h -1 , and then rebounded slightly, but basically stable at 4.46-4.70 μmol O 2 .mg -1 chl.h -1 between. When cc849 and lba were co-cultured with rhizobia respectively, the respiration rate of the system showed a rising trend, and w...

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Abstract

The invention, relating to the technique of biological hydrogen production, particularly relates to a method for increasing hydrogen yield of genetically modified Chlamydomonas reinhardtii by using rhizobium. The method is characterized by respectively mixing slow-growing Bradyrhizobium japomcum with genetically modified Chlamydomonas reinhardtii strain lba in the first stage and second stage of a two-step hydrogen producing method according to certain volume ratios to co-culture, wherein the genetically modified Chlamydomonas reinhardtii strain lba transforms leghemoglobin globulin subunit lba gene in Chlamydomonas reinhardtii strain cc849 and chloroplast. The results show that the slow-growing Bradyrhizobium japomcum promotes the growth of the Chlamydomonas reinhardtii strain lba in thefirst stage, and significantly increases the hydrogen yield of Chlamydomonas reinhardtii strain lba 17 times over.

Description

technical field [0001] The invention relates to biological hydrogen production technology, in particular to a method for improving the hydrogen production of Chlamydomonas reinhardtii by using brady-growing soybean rhizobia. Background technique [0002] Energy crisis and environmental pollution are prominent problems facing the world today. Hydrogen is a renewable bioenergy that is clean, has high combustion value, and can be utilized in various forms. [1] . Photosynthetic biological hydrogen production technology can directly convert solar energy into hydrogen energy. In particular, the substrate for photosynthetic hydrogen production by microalgae is water, which is rich in sources and is an ideal model for hydrogen production using solar energy. Moreover, the cultivation of microalgae is easy and does not occupy arable land. It is currently a research hotspot in the field of biological hydrogen production in the world. [2] . Chlamydomonas reinhardtii was selected as a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/89C12R1/41
Inventor 吴双秀
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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