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Anti-fatty acid synthetase polypeptide and its application

A fatty liver and amino acid technology, applied in the field of fatty acid synthase-resistant polypeptides, can solve the problems of unstable chemical properties, strong toxic side effects, etc., and achieve the effects of no immunogenicity, good water solubility, and obvious pharmacodynamic effects

Active Publication Date: 2012-10-24
TIANJIN TOPPHARM BIO SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these compound inhibitors have strong toxic and side effects as drugs, and some chemical properties are unstable, so they are subject to many restrictions in clinical application.

Method used

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  • Anti-fatty acid synthetase polypeptide and its application
  • Anti-fatty acid synthetase polypeptide and its application
  • Anti-fatty acid synthetase polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0088] Synthetic polypeptide functional fragment Anti-FAS-P18:

[0089] The present invention has synthesized the amino acid sequence of Gly-Gly-Cys-Arg-His-Lys-Leu-Val-Cys-Ala-Pro-Ala-Pro-Cys-Asn-Phe-Phe-Thr (SEQ ID NO: 1) polypeptide (hereinafter referred to as Anti-FAS-P18). The preparation of the polypeptide adopts a solid-phase synthesis method, such as using an AAPPTECAPex396 polypeptide synthesis instrument (purchased from Hong Kong Universal Analytical and Testing Instrument Co., Ltd.), in a closed explosion-proof glass reactor to make amino acids according to the sequence shown in SEQ ID NO: 1, From C-terminus-carboxyl-terminus to N-terminus-amino-terminus, this refers to the first amino acid sequence Gly-Gly-Cys-Arg-His-Lys-Leu-Val-Cys-Ala-Pro-Ala-Pro to be added to the amino acid sequence -The amino acid monomer of Cys-Asn-Phe-Phe-Thr is C-terminal Thr, then Phe, then Phe, until the last Gly and N-terminal Gly, continuously adding, reacting, synthesizing, operating...

Embodiment 2

[0097] Two methods are used to detect the anti-FAS activity of the polypeptide in Example 1 in vitro: the first method is to use molecular cloning technology to clone the cDNA expressing the polypeptide in Example 1 into the eukaryotic expression vector pcDNA3.1 On (+), through gene transfection, the purpose of expressing the studied polypeptide in liver cancer cells is achieved, and then the effect of the studied polypeptide on inhibiting FAS is observed; the second method is to use artificially synthesized polypeptides and directly add them to cultured liver cancer cells In the culture medium, observe the effect of polypeptide on inhibiting FAS.

[0098] The cells used in the experiment were liver cancer HepG2 cells. Since SREBP-1c is an upstream transcriptional regulator of FAS, by luciferase reporter gene detection method, detecting whether the promoter activity of SREBP-1c is inhibited at the molecular level can reflect the gene transcription of FAS. At the same time, th...

Embodiment 3

[0213] The HepG2 cells in the logarithmic growth phase were digested with trypsin to make a cell suspension, the number of cells was calculated, and the cells were diluted to 1×10 with sterile normal saline. 7 cells / ml and stored in ice water. Twelve 4- to 6-week-old female BALB / C nude mice were then randomly divided into 2 groups: ① Control group, subcutaneously inject 0.2ml of the above-mentioned diluted cells in the armpit of the right forelimb of each mouse, and only inject 0.5 ml sterilized distilled water (without polypeptide drugs); ② experimental group (administration dose is 10 mg / kg body weight). Subcutaneously inject 0.2ml of the above-mentioned diluted cells into the armpit of the right forelimb of each mouse, and after 7 days of injection, the tumor volume (V=L×W 2 ×0.5) up to 100mm 3 , and then subcutaneously inject the above polypeptide drugs (dissolve the freeze-dried polypeptide drugs with 0.5ml sterilized distilled water), inject once every two days, and in...

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Abstract

The invention relates to the polypeptide medicine field, and more specifically relates to the polypeptide of anti-fatty acid synthetase polypeptide (FAS) and polynucleotides for coding the polypeptide, and the application thereof. The invention relates to a polypeptide for inhibiting the fatty acid synthetase transcription and expression, the effects for inhibiting the fatty acid synthetase transcription and expression can be provided on the molecule level, cell level and animal overall level, thereby the overexpression of the fatty acid synthetase can be inhibited. The polypeptide and its peptide stimulant, which comprise the functional fragments and functional variants, and genes for coding the polypeptide, the peptide stimulant or the functional fragments and functional variants can be used for preventing and treating the tumors such as liver cancer, fatty liver and obesity which are relative to the fatty acid synthetase metabolism.

Description

technical field [0001] The invention relates to the field of polypeptide medicine, in particular to a polypeptide against fatty acid synthase, a polynucleotide encoding the polypeptide, and their applications. Background technique [0002] Fatty acid syntheses (FAS) are key enzymes in the process of endogenous fatty acid synthesis in organisms, which generate long-chain fatty acids by catalyzing acetyl-CoA and malonyl-CoA. FAS includes acetyltransferase (AT), malonyltransferase (MT), β-ketoacyl synthase (KS), β-ketoacyl reductase (KR), β-hydroxyacyl dehydratase (HD ), enoyl reductase (ER) and thioesterase (TE) and other seven functional domains. FAS is closely related to obesity and has higher expression in adipose tissue. At the same time, FAS is even more highly expressed in the human liver, and the fatty acid synthesis capacity of the liver is 8-9 times higher than that of adipose tissue. Therefore, FAS is also closely related to the formation of fatty liver. Fatty liv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/08C12N15/11C12N15/63C12N1/15C12N1/19C12N1/21C12N5/10A61K38/10A61K48/00A61P35/00A61P3/04A61P1/16
CPCC07K7/08C12N9/1029A61K38/00C12Y203/01085A61P1/16A61P3/04A61P35/00
Inventor 张晓东叶丽虹
Owner TIANJIN TOPPHARM BIO SCI & TECH CO LTD
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