Application of basic fibroblast growth factor to improvement of tissue engineered heart conduction bundle deformation

A technology of fibroblasts and growth factors, applied in the field of biomedical engineering, can solve problems such as the deformation of cell scaffold complexes, and achieve the effect of improving deformation problems

Inactive Publication Date: 2012-10-31
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to find a method to solve the deformation problem of the cell scaffold complex in construction

Method used

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  • Application of basic fibroblast growth factor to improvement of tissue engineered heart conduction bundle deformation
  • Application of basic fibroblast growth factor to improvement of tissue engineered heart conduction bundle deformation
  • Application of basic fibroblast growth factor to improvement of tissue engineered heart conduction bundle deformation

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Embryonic cardiac progenitor cells and collagen sponge scaffolds to construct tissue-engineered cardiac conduction bundles

[0030] Referring to Zhang Xi et al. (ZHANG Xi, ZHANG Chuan-Sen, LIU Yan-Chun, et al. Isolation, Culture and Characterization of Cardiac Progenitor Cells Derived from Human Embryonic Heart Tubes. Cells Tissues Organs. 2009; 190(4): 194- 208.) method to isolate and culture SD rat embryonic cardiac progenitor cells. Embryonic cardiac progenitor cells were harvested at 1 x 10 7 density per ml, planted in (1.5×0.2×0.5cm 3 ) Collagen sponge scaffold material (Wuxi Bedi Biological Co., Ltd.), 20% high-glucose medium (fetal bovine serum and anti-penicillin-streptomycin from Gibico company, high-glucose medium from Hyclone company) was routinely cultured, and the medium was changed every three days .

[0031] See the article "Preliminary Construction of Tissue-Engineered Cardiac Conduction Bundle" for various histomorphological identification and pe...

Embodiment 2

[0037] Embryonic cardiac progenitor cells were harvested at 1 x 10 7 The density of each / ml is planted in (1.5×0.2×0.5cm 3 ) on the collagen sponge scaffold material. The complexes were cultured in ordinary culture medium for two days, and then the culture medium was changed according to the experimental groups. The groups were as follows: RM: common culture medium group, bFGF group: common culture medium + bFGF (concentration: 10 ng / ml) (Peprotech Company). Change the medium every three days.

[0038] The photos of the complexes are taken regularly, and the photos are taken vertically, and the deformation of the complexes is reflected by the change of the vertical projected area of ​​each complex. At a concentration of 10ng / ml, bFGF did not improve the deformation of the constructed tissue-engineered cardiac conduction bundle.

[0039] The results showed that the bFGF with a concentration of 10ng / ml did not improve the deformation of the complex, as figure 2 shown.

Embodiment 3

[0041] Embryonic cardiac progenitor cells were harvested at 1 x 10 7 The density of each / ml is planted in (1.5×0.2×0.5cm 3 ) on the collagen sponge scaffold material. The complexes were cultured in ordinary culture medium for two days, and then the culture medium was changed according to the experimental groups. Grouped as follows: RM: common culture medium group, bFGF group: common culture medium + bFGF (concentration: 50 ng / ml) (Peprotech Company). Change the medium every three days.

[0042] The photos of the complexes are taken regularly, and the photos are taken vertically, and the deformation of the complexes is reflected by the change of the vertical projected area of ​​each complex. At a concentration of 50ng / ml, bFGF can effectively improve the deformation of the constructed tissue-engineered cardiac conduction bundle.

[0043] The results showed that: at the bFGF concentration of 50ng / m, the contraction degree of the complex in the bFGF group was obviously small...

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Abstract

The invention relates to the technical field of biomedical engineering. The inventor builds a tissue engineered conduction bundle which consists of an embryonic heart progenitor cell and a collagen sponge stent and has very high compatibility. As proved by in-vitro researches, the built tissue engineered conduction bundle tends to undergo contraction deformation, and an initially-designed stent material form cannot be maintained accurately. The invention aims to find a method for solving the problem of deformation of a built cell stent compound. The invention provides application of a basic fibroblast (bFGF) growth factor to improvement of tissue engineered heart conduction bundle deformation. A basic fibroblast growth factor is added into a culture fluid of a tissue engineered heat conduction bundle, so that the problem of deformation of the tissue engineered heart conduction bundle is improved, a tissue engineered heart conduction bundle which can keep a better original shape in vitro and has electrocardio-conduction property is obtained, and a basis is laid for the treatment of an arrhythmi disease caused by atrioventricular conduction stagnation.

Description

technical field [0001] The invention relates to the technical field of biomedical engineering, in particular to the application of basic fibroblast growth factor in improving the deformation of tissue-engineered cardiac conduction bundles. Background technique [0002] Cardiac atrioventricular block is one of the common arrhythmia diseases. At present, the main method for treating cardiac atrioventricular block is to place an electronic cardiac pacemaker. Although the effectiveness of current electronic pacemaker therapy is beyond doubt, for children, long-term electronic pacing therapy has serious risks and complications. Therefore, it is imperative to seek safe and effective treatment methods. The proposal, establishment and development of tissue engineering provide a new way to solve this problem. The basic principle of tissue engineering is to expand a small amount of seed cells in vitro and compound them with biomaterials to construct new tissues or organs, which are ...

Claims

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Application Information

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IPC IPC(8): A61L27/38A61L27/24
Inventor 张传森李晓童张喜
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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