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A kind of Grifola frondosa galactan and preparation method thereof

A technology of galactan and Grifola frondosa is applied in the directions of anti-tumor drugs, drug combinations, allergic diseases, etc., and can solve the problems such as the separation of galactan that has not been seen, and achieves the improvement of the immune activity of organisms, which is time-consuming. short, immune-enhancing effect

Inactive Publication Date: 2016-12-21
SHANGHAI ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no research report on the isolation and acquisition of biologically active galactan at home and abroad.

Method used

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  • A kind of Grifola frondosa galactan and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Submerge 1 kg of Grifola frondosa sporocarp with 10 times of water, keep it warm for 2 hours at 80°C, repeat 4 times, centrifuge the extract at 5000rpm / min, and then perform ultrafiltration with a 100,000 ultrafiltration membrane to collect the retained part Precipitate the retentate with 10% ethanol, dissolve the precipitate with distilled water, and go through ion column chromatography (the mobile phase is 50% sodium chloride), collect the first main peak part of the salt phase, and then pass through a 1 million ultrafiltration membrane Ultrafiltration, desalination, concentration, and finally freeze-drying can obtain about 10 g of Grifola frondosa galactan, which was determined by high-performance liquid phase method, wherein more than 90% of Grifola frondosa galactan has a molecular weight of more than 5.9 million.

Embodiment 2

[0037] Use 20 times of water to immerse 1 kg of Grifola frondosa fruiting body, keep it warm for 1 hour at 90°C, and repeat 3 times. After the extract is centrifuged at 5000 rpm / min, it is ultrafiltered with a 500,000 ultrafiltration membrane to collect the retained part ; Precipitate the retentate with 20% ethanol, dissolve the precipitate with distilled water, and go through ion column chromatography (mobile phase is 30% sodium chloride), collect the first main peak part of the salt phase, and then pass through a 750,000 ultrafiltration membrane Ultrafiltration, desalination, concentration, and finally freeze-drying can obtain about 15 g of Grifola frondosa galactan, of which the molecular weight of Grifola frondosa galactan is more than 90%.

Embodiment 3

[0039] Use 40 times of water to immerse the fruiting bodies of Grifola frondosa, keep warm for 1 hour at 100°C, and repeat twice. After the extract is centrifuged at 5000 rpm / min, it is ultrafiltered with a 750,000 ultrafiltration membrane to collect the retained part; Precipitate the retentate with 30% ethanol, dissolve the precipitate with distilled water, and collect the first main peak part of the salt phase through ion column chromatography (mobile phase is 10% sodium chloride), and then pass through a 500,000 ultrafiltration membrane Filter and desalinate, concentrate, and finally freeze-dry to obtain about 15 g of Grifola frondosa galactan, of which the molecular weight of Grifola frondosa galactan is more than 90%.

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Abstract

The invention provides grifola frondosa galactan and a preparation method thereof. The grifola frondosa galactan is obtained by the steps of hot water leaching, ultrafiltration, alcohol precipitation, ion column chromatography, re-ultrafiltration and freeze-drying. The grifola frondosa galactan has high purity up to more than 90%, contains more than 90% of components with a molecular weight of more than 5.9 million Daltons, and serves the effect of inhibiting the tumor growth.

Description

technical field [0001] The invention relates to the field of edible and medicinal fungi, in particular to a grifola frondosa galactan and a preparation method thereof. Background technique [0002] Grifola frondosa is an edible and medicinal fungus, and its biologically active components are mainly polysaccharides. At present, domestic and foreign research results on its separation show that the active ingredients of Grifola frondosa are mainly 3-glucan, proteoglucan, heteropolysaccharides, etc., with a molecular weight of less than 2 million Daltons, and have immune activity and tumor-inhibiting activity. . However, there are no research reports on the isolation and acquisition of biologically active galactan at home and abroad. Invention content: [0003] The present invention firstly provides a kind of Grifola frondosa galactan, the preparation principle of which is: first extract the fruit body of Grifola frondosa with hot water, and then comprehensively use high mol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/02A61P35/00
Inventor 周昌艳乔彦茹唐庆九刘艳芳杨焱贾薇周帅吴迪张劲松吴爱忠
Owner SHANGHAI ACAD OF AGRI SCI