Proliferation accelerator for human adipose-derived stem cells and application method thereof

A technology of human adipose stem cells and application methods, which is applied in the field of human adipose stem cell proliferation promoters, and can solve the problems of slow subculture proliferation, complicated methods, and small number of stem cells

Active Publication Date: 2012-10-31
JIANGSU RE STEM BIOTECH
View PDF4 Cites 25 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method of cultivating stem cells is that the method is complicated, the numb

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Proliferation accelerator for human adipose-derived stem cells and application method thereof
  • Proliferation accelerator for human adipose-derived stem cells and application method thereof
  • Proliferation accelerator for human adipose-derived stem cells and application method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: Common human adipose stem cell basal medium preparation:

[0031] Take 900 mL of DMEM / F12 cell culture medium (purchased from TAKARA BIO INC.) and add antibiotics: 90000 U of penicillin and 90000 U of streptomycin.

[0032] Adjust pH value: use mass fraction 5% NaHCO 3 Adjust the pH to 7.2.

[0033] Sterilization by filtration: Use a filter membrane with a pore size of 0.45 μm and 0.22 μm, the upper layer is 0.45 μm, and the lower layer is 0.22um to ensure the filtering effect.

[0034] Add calf serum: before use, add calf serum to make up to 1000mL (purchased from TAKARA BIO INC.).

Embodiment 2

[0035] Example 2: Preparation of a proliferation medium A containing human adipose stem cell proliferation promoter

[0036] If the predetermined volume is 1000mL, take 900mL of DMEM / F12 cell culture medium (TAKARA BIO INC.), add antibiotics: penicillin 90000U, streptomycin 90000U.

[0037] Adjust pH value: use mass fraction 5% NaHCO 3 Adjust the pH to 7.2.

[0038] Adding proliferation promoter: Weigh the components and amounts shown in Table 1 and add them to the prepared DMEM / F12 cell culture medium in sequence, shake or sonicate to help dissolve, and do not heat to help dissolve.

[0039] Table 1

[0040]

[0041] Then the concentration of each component shown in the above table in proliferation medium A is insulin 0.1 μg / mL, hydrocortisone 10 -10 mol / L, parathyroid hormone 2×10 -10mol / L, estradiol 0.2ng / mL, testosterone 0.8ng / mL, platelet-derived growth factor-AB 1ng / mL, epidermal growth factor 2ng / mL, basic fibroblast growth factor 2ng / mL, L-glutamine Amide 0.4mm...

Embodiment 3

[0044] Example 3: Preparation of a proliferation medium B containing human adipose stem cell proliferation promoter

[0045] The preparation method and predetermined amount are as in Example 2, but when weighing, the components in Table 1 can be used to make the concentrations in the proliferation medium B successively insulin 10 μg / mL, hydrocortisone 5×10 -9 mol / L, parathyroid hormone 7×10 -10 mol / L, estradiol 3.5ng / mL, testosterone 15ng / mL, platelet-derived growth factor-AB8ng / mL, epidermal growth factor 16ng / mL, basic fibroblast growth factor 20ng / mL, L-glutamine 4mmol / L, 2-mercaptoethanol 0.15mmol / L, leukemia inhibitory factor 7ng / mL, L-ascorbic acid 80μg / mL, biotin 45μg / mL, dexamethasone 5×10 -8 mol / L, IGF-I10ng / mL and SCF15ng / mL were weighed.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a proliferation accelerator for human adipose-derived stem cells and an application method thereof. The proliferation accelerator for the human adipose-derived stem cells comprises insulin, hydrocortisone, parathyroid hormone, estradiol, testosterone, a platelet-derived growth factor-AB, an epidermal growth factor, a basic fibroblast growth factor, L-glutamine, 2-mercaptoethanol, a leukemia inhibitory factor, L-ascorbic acid, biotin, dexamethasone, an IGF-I (Insulin-like Growth Factor-I) and a SCF (Stem Cell Factor). The proliferation accelerator for the human adipose-derived stem cells, disclosed by the invention, is applied to proliferation and subculture of the adipose-derived stem cells, can be used to not only specifically promote the growth sped and the proliferation quantity of the adipose-derived stem cells to increase in the form of geometric progression, but also suppress early differentiation and maturity of the adipose-derived stem cells so as to prevent apoptosis or premature senility of the stem cells during culturing and keep the vigorous and active differentiation potentials of the adipose-derived stem cells. The proliferation accelerator for the human adipose-derived stem cells, disclosed by the invention, has good application prospect in the field of culture of the adipose-derived stem cells.

Description

technical field [0001] The invention relates to the fields of biotechnology and cell engineering, in particular to a human adipose stem cell proliferation promoter. Background technique [0002] Human adipose-derived stem cells (ADSCs), also known as human adipose-derived stem cells (ADSCs), are currently a type of adult stem cells widely used in the fields of tissue engineering and regenerative medicine, and have the same multi-directional differentiation potential as bone marrow mesenchymal stem cells. ADSCs showed fibroblast-like growth, abundant cytoplasm and nucleoli, arranged in parallel or swirl-like arrangement. Cell cycle analysis showed that 69% of cells were in G0 / G1 phase, 24% in S phase, and 8% in G2 / M phase. The cells were subcultured for 2-3 days in the presence of fetal bovine serum to double the cell proliferation. After multiple passages (10-20 passages), the cell proliferation rate did not slow down significantly. Senescent cells appeared in the cell pop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12N5/0775
Inventor 焦阳
Owner JIANGSU RE STEM BIOTECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap