Method for producing somatic cell cloned bovine blastocyst

A technology of somatic cell cloning and donor cells, applied in biochemical equipment and methods, botany equipment and methods, applications, etc., can solve the problem of low efficiency of somatic cell cloning
CN102766655AInactive Publication Date: 2012-11-07INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN Β· China
Current Assignee / Owner
INST OF ANIMAL SCI OF CHINESE ACAD OF AGRI SCI
Publication Date
2012-11-07
Estimated Expiration
Not applicable Β· inactive patent

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Abstract

he invention discloses a method for producing somatic cell cloned bovine blastocysts. According to the method, to bovine ear limbal fibroblasts are used as nuclear transfer donorcells, and mature bovine oocytes cultivated in vitro are used as nuclear transfer receptor cells; the oocytes are denucleated by extrusion and removed with the zona pellucida; the oocytes and the donor cells are adhered in an arrangement way of oocytes-donor cells-oocytes and treated with electro-fusion to obtain reconstructed embryos; and the reconstructed embryos are activated and cultured in vitro to obtain the cloned blastocysts. The method can improve the production efficiency of the cloned bovine blastocysts.
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Description

Technical field

[0001] The invention relates to a method for producing somatic cell cloned bovine blastocysts. Background technique

[0002] Mammalian somatic cell cloning refers to the transfer of somatic cell nuclei into enucleated recipient cells (mature oocytes or fertilized eggs), and new reconstructed embryos are reprogrammed, divided, differentiated and developed according to the genetic information of the donor. The more popular methods of somatic cell nuclear transfer are manual cloning and micromanipulation.

[0003] The efficiency of cloning technology itself is affected by various links, including the enucleation of oocytes, somatic cell injection into oocytes, fusion of enucleated oocytes and somatic cells, activation of reconstructed embryos, and in vitro culture of reconstructed embryos.

[0004] During the cloning process, the denucleation of mature oocytes is an important part of nuclear transfer technology, which requires complete removal while minimizing damage to...

Claims

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