Gene VIb subtype Rubulavirus Newcastle disease virus attenuated strain VIbI4 and construction method thereof

A technology of Newcastle disease virus and construction method, which is applied in the field of gene VIb subtype Newcastle disease virus attenuated strain VIbI4 and its construction, can solve problems such as inconsistency of genotypes of epidemic strains, and achieve the effect of high reproduction performance
CN102776156AActive Publication Date: 2012-11-14YANGZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
YANGZHOU UNIV
Publication Date
2012-11-14

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Abstract

The invention relates to gene VIb subtype Avian pneumo-encephalitis virus attenuated strain VIbI4 and a construction method thereof. The preserving number of the gene VIb subtype scriber set Avian pneumo-encephalitis virus attenuated strain VIbI4 is CGMCCNo: 6149. The gene VIb subtype Rubulavirus Newcastle disease virus attenuated strain VIbI4 and the construction method thereof relate to a technology for applying reverse genetics; and the method comprises the following steps: carrying out mutation on an F gene by using a transcription carrier Ptvt / 071204 containing Avian pneumo-encephalitis virus JS / 07 / 04 / Pi full gene genome from pigeons so as to obtain to a transcription carrier ApTVT / 071204, and then substituting corresponding parts on the ApTVT / 071204 by the NP, P and L genetic fragments of Avian pneumo-encephalitis virus rNDV / I4 so as to obtain recombinant plasmids ApTVT / VIbI4, wherein the plasmids successfully save a recombinant virus VIbI4 after transfecting a BSR-T7 / 5 cell together with auxiliary plasmids. The virus is relatively high in propagating titre, is suitable for large-scale production of vaccines and can be used for manufacturing vaccines.
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Description

technical field

[0001] The invention relates to the application of reverse genetic technology to produce a gene VIb subtype Newcastle disease virus attenuated strain VIbI4 for making vaccines. Background technique

[0002] Reverse genetics manipulation technique (Reverse genetics manipulation technique) refers to the construction of infectious molecular clones of RNA viruses in vitro, the reverse transcription of viral genomic RNA into cDNA, and various in vitro artificial manipulations on the DNA molecular level. Genomic cDNA and various auxiliary proteins to assemble a new RNA virus [Leyrer S, Neubert WJ, SedlmeierR. Rapid and efficient recovery of Sendai virus from cDNA: factors influencing recombinant virus rescue. J Virol Methods 1998, 75( 1):47-58.], also known as full-length infectious cDNA cloning technology, and often referred to as "virus rescue". Since the "rescued" RNA virus is derived from cDNA clones, various in vitro artificial operations can be performed on ...

Claims

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