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Production method for xylanase

A technology of xylanase and production method, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of high production cost, influence on promotion, large investment in liquid submerged fermentation method, etc.

Inactive Publication Date: 2014-07-02
BAIYIN SINO BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the liquid submerged fermentation method requires a large investment and high production cost, in which the main raw material accounts for 65% or more of the production cost. Due to the high production cost of xylanase, the product price is high, which directly affects market development and product application. to promote

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  • Production method for xylanase

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specific Embodiment approach

[0030] detailed description See accompanying drawing: embodiment 1

[0031] 1. Transfer the Aspergillus niger strain test tube slant strain under aseptic conditions into a 5-liter triangular culture flask with a prepared medium, pH 5.3, and sterilize at 122°C for 30 minutes; Cultivate for 30 hours under the condition of ℃ to obtain the Aspergillus niger strain for subsequent use;

[0032] Wherein the culture medium is: mass percent is 1.5% glucose, 1.5% potassium dihydrogen phosphate, 0.5% ammonium sulfate, 0.1% magnesium sulfate, 0.05% sodium chloride, 2% corn steep liquor, and the balance is water;

[0033] 2、 6m3 seed tank expansion culture: the medium is: 1% glucose, 0.8% lactose, 1% potassium dihydrogen phosphate, 0.3% ammonium sulfate, 0.1% magnesium sulfate, 0.05% calcium chloride, 1% corn industrial grade Put the raw materials into the seed tank, adjust the volume to 3 cubic meters with tap water, stir evenly, then directly sterilize with steam, keep warm at 12...

Embodiment 2

[0039] 1、 The culture condition of the 5 liters of triangular culture bottles of prepared culture medium is pH5.5, and 120 ℃ of insulations are sterilized for 30 minutes, and the rotating speed of shaker is 200rpm, and 29 ℃ of cultures 30 hours, get the Aspergillus niger bacterial classification for subsequent use; All the other are with embodiment 1 same;

[0040] 2、 Expanded culture of 6m3 seed tank strains: direct steam sterilization, heat preservation at 121°C for 30 minutes, then lower the temperature to 32°C, and insert 5 bottles of Aspergillus niger strains in 5-liter triangular flasks, at an air volume ratio of 1:1.15 and a temperature of 29 Cultivate for 29 hours under the condition of 0.065Mpa at a tank pressure of 0.065Mpa; the rest are the same as in Example 1;

[0041] 3. Steam direct sterilization, heat preservation at 117°C for 30 minutes, then lower the temperature to 31°C, insert 6m3 seed tanks to expand the cultivation of Aspergillus species, under the con...

Embodiment 3

[0045] 1、 The culture condition of the 5 liters of triangular culture bottles of prepared culture medium is pH5.3, and 124 ℃ of insulations are sterilized for 30 minutes, and the rotating speed of shaker is 195rpm, and 28.5 ℃ of cultures 31 hours, get the aspergillus niger strain for subsequent use; All the other and embodiment 1 same;

[0046] 2、 6m3 seed tank expansion culture:

[0047] Sterilize directly by steam, keep warm at 123°C for 30 minutes, then cool down to 31°C, put five bottles of Aspergillus niger strains in 5-liter triangular flasks, under the conditions of air volume ratio 1:1.12, temperature 29.5°C, tank pressure 0.05Mpa Cultivate 29.5 hours standby; All the other are identical with embodiment 1;

[0048] 3. Steam direct sterilization, keep warm at 118°C for 30 minutes, then cool down to 30°C, insert 6m3 seed tanks to expand the cultivation of Aspergillus species, under the conditions of air volume 1:1.13, temperature 29°C, tank pressure 0.07Mpa Cultiva...

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Abstract

A method for producing xylanase, which uses Asperillus niger as a culture medium, uses lactose as the main raw material, and adopts a liquid submerged fermentation method to produce xylanase, which is characterized in that: continuous supplementary fermentation culture is added during the fermentation process base, and intermittently release the fermented mash from the fermenter in one fermentation cycle; the obtained fermented mash is filtered through a plate and frame to obtain a xylan enzyme liquid, which is then concentrated by ultrafiltration and spray-dried to obtain a high-activity xylan Enzyme powder product. The invention solves the problems of reducing the production cost of xylanase and improving the yield. The present invention adopts the combination of high-efficiency and continuous addition of fermentation culture materials and intermittent mash-out method; under the condition of inputting the same amount of raw materials, the xylanase fermented liquid produced by unit fermentation volume per hour increases by 71.9%, and the total output increases by about 29%. %, the unit raw material cost is reduced by about 22%.

Description

technical field [0001] The invention belongs to the technical field of production of biological enzyme preparations, in particular to a production method of xylanase. Background technique [0002] Xylanase is a non-starch hydrolytic enzyme that hydrolyzes (1→4)-β glycosidic bonds in xyloglucan (or pentosan) molecules, degrades them into small molecules, reduces water holding capacity and viscosity, and is widely used It is widely used in industries such as papermaking, feed, food, medicine, and environmental protection. With the rapid development of the national economy, it has become more and more widely used. [0003] Xylanase is mainly produced by microbial fermentation; usually solid fermentation and liquid submerged fermentation. The solid fermentation method was applied earlier, and has been gradually replaced by the liquid submerged fermentation method due to its high labor intensity, difficult control, unstable product quality, especially hygienic indicators; th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12R1/685
Inventor 孙维国李统峰丁少明
Owner BAIYIN SINO BIOTECH