Method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis

A technology of xylo-oligosaccharide and directional enzymolysis, applied in the direction of fermentation, can solve the problems of inability to directionally degrade xylo-oligosaccharide, increase the load of xylo-oligosaccharide production and refining process, etc. Consistency and economic efficiency, the effect of simplifying the extraction process

Inactive Publication Date: 2011-06-01
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, steam explosion cannot degrade xylan in a directional manner to generate xylo-oligosaccharides with a suitable degree of polymerization. At the same time, excessive steam explosion will also produce many degradation products, such as furfural, hydroxymethylfurfural and lignin derivatives, which increase the The load of xylo-oligosaccharide production and refining process

Method used

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  • Method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis
  • Method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis
  • Method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis

Examples

Experimental program
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Effect test

Embodiment 1

[0030] Air-dried corncobs are pretreated by manual sorting to remove mold, vibrating screen for sand removal and iron removal, and then mechanically crushed until the length of the particles does not exceed 50mm and the diameter does not exceed 20mm.

[0031] Add materials to the intermittent or continuous automatic steam blaster, open the steam inlet valve through the automatic control system and directly pass in 2.0MPa supersaturated steam to quickly heat the materials to 200~205°C, and automatically open the discharge valve instantaneously after maintaining for 3~8min Create a blasting effect and get blasting material. The blasting material was tested, and the recovery rate of the total solids of the blasting material reached over 80.0%, the pH value was 4.5-5.0, and the recovery rate of xylan was higher than 70.0%, of which soluble xylan and xylooligosaccharides accounted for 36.0% ~38.0%, the yield of xylo-oligosaccharide components with a degree of polymerization of 2-6 ...

Embodiment 2

[0033] The directional enzymatic hydrolysis of blasting materials is carried out by a mechanical stirring enzyme hydrolysis device. Add an appropriate amount of soft water to adjust the total solids concentration of the blasting material to 5.0-10.0% (w / w), add endoxylanase according to the amount of 5-15U / g xylan, and control the reaction temperature at 50±2°C and The stirring rate is 50-100 rpm, and the enzymolysis solution is obtained after 20-24 hours of directional enzymolysis reaction. The enzymolysis solution was tested, and during the enzymolysis process, the enzymolysis yield of xylooligosaccharide components with a degree of polymerization of 2 to 6 exceeded 23.5%. Taking the xylan contained in the corncob steam explosion material as the calculation basis, the total yield of xylooligosaccharides with a polymerization degree of 2-6 after steam explosion and directional enzymatic hydrolysis is 30.0%.

Embodiment 3

[0035] Under the condition of 40-45°C, the plate and frame filter is used to press filter the enzymatic hydrolysis system to remove solid impurities. The inlet pressure of the plate and frame filtration operation is controlled at 1.0-1.5MPa, and the filter cake is washed with 20-25% (w / w) soft water to obtain xylo-oligosaccharide filtrate; Module (membrane pore size 0.20 ~ 0.45 μm) to filter xylooligosaccharide filtrate to obtain clarified liquid, control the operating pressure 0.15 ~ 0.20MPa; under the condition of 25 ~ 30 ℃, then use hollow fiber ultrafiltration membrane module (molecular weight cut off 20000 Dalton ) to further separate and purify the clarified liquid to obtain dilute xylo-oligosaccharide sugar liquid, and control the operating pressure to be 0.30-0.35 MPa.

[0036] Under the condition of 25 ~ 30 ℃, control the appropriate flow rate so that the dilute xylooligosaccharide liquid flows through the weakly basic anion exchange chromatography column and the macr...

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Abstract

The invention discloses a method for preparing xylo-oligosaccharides by adopting steam explosion and oriented enzymolysis. The method comprises the following steps of plant fiber raw material chopping, steam explosion, endo-xylanase oriented enzymolysis, filter pressing by an enzymolysis system, micro-filtration, ultrafiltration, separation and extraction, anion exchange resin refining, macroporous phenolic aldehyde absorbent resin refining and decompressed evaporation concentration. The method for preparing xylo-oligosaccharides, provided by the invention, realizes that steam explosion and oriented enzymolysis are organically combined to substitute the traditional acid or alkali extraction method or single steam explosion method, thereby not only effectively degrading and dissolving out xylan in raw materials, but also overcoming the defects of more consumption and various impurities of acid or alkali chemicals, particularly overhigh content of noxious substances and high cost for following separating and refining in other processes, realizing no waste water emission in the production process, greatly simplifying the extraction process of the xylan, saving the operation labor hour, and remarkably improving the environment friendless and the economic benefit of production of xylo-oligosaccharides.

Description

technical field [0001] The invention relates to a preparation method of xylooligosaccharides, in particular to a method for preparing xylooligosaccharides by steam explosion-directed enzymatic hydrolysis. Background technique [0002] Xylooligosaccharide is a general term for a class of oligosaccharides linked by β-1,4-xylosidic bonds with a low degree of polymerization. The effect of mycofactor and immune enhancer can effectively regulate the intestinal microecology and activate the immune function of the body and other health-care and growth-promoting functions. It can be widely used in medicine, food, beverage and feed and other related industries. [0003] At present, the production of xylo-oligosaccharides mainly adopts the method of alkali extraction or acid extraction of plant cellulose, and then xylan is catalyzed by xylanase to hydrolyze xylan to produce xylo-oligosaccharides. A certain amount of acid and alkali chemicals need to be consumed in these processing pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
Inventor 徐勇余世袁勇强朱汉静范丽朱汉生
Owner NANJING FORESTRY UNIV
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